Detection of target analytes using particles and electrodes

• US 20070059732A1
• Filed: 06/01/2006
• Published: 03/15/2007
• Est. Priority Date: 10/27/1998
• Status: Abandoned Application

• Current Assignee: Roche Molecular Systems (Roche Holding AG)
• Original Assignee: Clinical Micro Sensors Inc. (Roche Holding AG)
• Inventors: Mucic, Robert, Bamdad, Cynthia
• Application Number:
  US11/445,815
• Publication Number:
  US 20070059732A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01J 13/00 Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: C12Q 2525/197 incorporating a spacer/coupling moiety
• CPC Class Codes: C12Q 2525/313 Branched oligonucleotides
• CPC Class Codes: C12Q 2563/113 the label being electroactive, e.g. redox labels
• CPC Class Codes: C12Q 2565/101 Interaction between at least two labels
• CPC Class Codes: C12Q 2565/1015 labels being on the same oligonucleotide
• CPC Class Codes: G01N 27/3275 Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
• CPC Class Codes: G01N 27/3277 being a redox reaction, e.g. detection by cyclic voltammetry voltammetry per se G01N27/42, G01N27/48
• CPC Class Codes: G01N 27/48 using polarography, i.e. measuring changes in current under a slowly-varying voltage
• CPC Class Codes: Y10S 977/773 Nanoparticle, i.e. structure having three dimensions of 100 nm or less
• CPC Class Codes: Y10S 977/92 Detection of biochemical
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

The invention relates to the use of particles comprising binding ligands and electron transfer moieties (ETMs). Upon binding of a target analyte, a particle and a reporter composition are associated and transported to an electrode surface. The ETMs are then detected, allowing the presence or absence of the target analyte to be determined.

Claims

1-6. -6. (canceled)

7. A method of detecting cells using colloid particles, comprising:

a) forming an assay complex on a surface, wherein said assay complex comprises

1) said cells;

2) at least one colloid particle comprising a binding ligand; and

3) at least one signaling moiety that is either directly or indirectly attached to said assay complex; and

b) detecting said cells by detecting said signaling moiety.

8. A method according to claim 7, wherein said surface is an electrode.

9. A method according to claim 7, wherein said colloid particle is a gold colloid particle.

10. A method according to claim 7, wherein said colloid particle further comprises a self-assembled monolayer (SAM).

11. A method according to claim 10, wherein said SAM is a mixed SAM comprising a at least two species of molecules.

12. A method according to claim 10, wherein said binding ligand is attached to said SAM via a transitional metal ligand.

13. A method according to claim 12, wherein said transitional metal ligand comprises nickel.

14. A method according to any of claims 7 to 13, wherein said binding ligand is a protein.

15. A method according to claim 7, wherein said signaling moiety is an electron transfer moiety (ETM).

16. A method according to claim 7, wherein said signaling moiety is a fluorescent moiety.

17. A composition comprising a colloid particle comprising:

1) at least one signaling moiety; and

2) a mixed self-assembled monolayer (SAM), wherein said SAM comprises at least two species of molecules.

18. A composition according to claim 17, wherein one of said species of molecules comprises a protein.

19. A composition according to claim 17, wherein one of said species of molecule comprises an insulator.

20. A composition according to claim 19, wherein said insulator comprises an alkyl or a heteroalkyl oligomer.

21. A composition according to claim 17, wherein said signaling moiety comprises an electron transfer moiety (ETM).

22. A composition comprising a colloid particle comprising:

a) a self-assembled monolayer (SAM); and

b) a non-DNA binding ligand attached to said colloid particle.

23. A composition according to claim 22, wherein said binding ligand comprises a protein.

24. A composition according to claim 23, wherein said protein is a peptide.