Fluorescence energy transfer probes with stabilized conformations
First Claim
Patent Images
1. A compound having a structure selected from:
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X—
R-A-Q-(Y)n, R—
X-A-(Y)n-Q, R—
X-A-Q-(Y)n, and
X—
R-A-(Y)n-Q wherein, A is a nucleic acid chain comprising nucleic acid monomers selected from the group consisting of natural nucleic acids, modified nucleic acids and combinations thereof;
R is a molecular energy transfer donor;
Q is a molecular energy acceptor; and
X and Y are the same or different and are non-nucleic acid stabilizing moieties that interact to bring R and Q into operative proximity, thereby enabling transfer of energy from R to Q; and
n is 0 or 1.
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Abstract
The present invention provides a class of Conformationally Assisted Probes comprising (a) a nucleic acid moiety; (b) an energy donor moiety; (c) an energy acceptor moiety; and (d) one or more stabilizing moieties.
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Citations
31 Claims
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1. A compound having a structure selected from:
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X—
R-A-Q-(Y)n, R—
X-A-(Y)n-Q, R—
X-A-Q-(Y)n, and
X—
R-A-(Y)n-Qwherein, A is a nucleic acid chain comprising nucleic acid monomers selected from the group consisting of natural nucleic acids, modified nucleic acids and combinations thereof;
R is a molecular energy transfer donor;
Q is a molecular energy acceptor; and
X and Y are the same or different and are non-nucleic acid stabilizing moieties that interact to bring R and Q into operative proximity, thereby enabling transfer of energy from R to Q; and
n is 0 or 1. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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- 19. A compound having the formula:
- 26. A compound having the structure:
Specification