Neuronal differentiation of stem cells
First Claim
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1. A method for inducing stem cells to differentiate into neuronal cells comprising:
- a) culturing said stem cells with basic fibroblast growth factor;
b) culturing the cells of step a) with fibroblast growth factor 8 and Sonic Hedgehog;
c) culturing the cells of step b) with brain-derived neurotrophic factor;
d) co-culturing the cells of step c) with astrocytes.
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Abstract
The present invention relates to compositions and methods for culturing stem cells, such that neuronal differentiation can be achieved.
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Citations
13 Claims
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1. A method for inducing stem cells to differentiate into neuronal cells comprising:
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a) culturing said stem cells with basic fibroblast growth factor;
b) culturing the cells of step a) with fibroblast growth factor 8 and Sonic Hedgehog;
c) culturing the cells of step b) with brain-derived neurotrophic factor;
d) co-culturing the cells of step c) with astrocytes. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method for inducing cells to differentiate into neuronal cells comprising co-culturing the cells with astrocytes, said cells having gone through the steps of:
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a) culturing stem cells with basic fibroblast growth factor;
b) culturing the cells of step a) with fibroblast growth factor 8 and Sonic Hedgehog;
c) culturing the cells of step b) with brain-derived neurotrophic factor.
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Specification