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Neuronal differentiation of stem cells

  • US 20070059823A1
  • Filed: 07/02/2004
  • Published: 03/15/2007
  • Est. Priority Date: 07/02/2003
  • Status: Active Grant
First Claim
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1. A method for inducing stem cells to differentiate into neuronal cells comprising:

  • a) culturing said stem cells with basic fibroblast growth factor;

    b) culturing the cells of step a) with fibroblast growth factor 8 and Sonic Hedgehog;

    c) culturing the cells of step b) with brain-derived neurotrophic factor;

    d) co-culturing the cells of step c) with astrocytes.

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