Readhead for optical inspection apparatus

• US 20070064220A1
• Filed: 06/03/2004
• Published: 03/22/2007
• Est. Priority Date: 06/03/2003
• Status: Active Grant

• Current Assignee: Siemens Healthcare Diagnostics Incorporated (Siemens AG)
• Original Assignee: Bayer Healthcare LLC (Bayer AG)
• Inventors: Ross, Gary, Ledden, David, Stock, Nicholas
• Granted Patent: US 7,499,154 B2
• Time in Patent Office: Days
• US Class Current: 356/73
• CPC Class Codes: G01N 21/251 Colorimeters; Construction thereof
• CPC Class Codes: G01N 21/64 Fluorescence; Phosphorescence
• CPC Class Codes: G01N 21/8483 Investigating reagent band test-element handling not specific to a test method G01N33/4875; analytical elements specific to chemical analysis of biological material G01N33/52; autometer with reagent band G01N35/04

Abstract

A readhead for illuminating a sample carrier and receiving light from the sample carrier, including a housing for receiving a sample carrier, an array of light sources mounted within the housing in a fixed position relative to the sample carrier, and including first and second light-emitting diodes for emitting substantially monochromatic light of a two different wavelengths, a light guide mounted in the housing between the light-emitting diodes and the sample carrier, and a light detector coupled to receive light from the sample carrier. The readhead also includes a light source for directing excitation light of a predetermined wavelength to the sample carrier, and a light filter positioned between the sample carrier and the light detector and adapted to prevent passage therethrough of the excitation light. The readhead allows both fluorescence spectroscopy and reflectance spectroscopy to be conducted on the sample carrier.

Claims

1. A readhead for a diagnostic instrument for illuminating a sample carrier and receiving light from the sample carrier, the readhead comprising:

a housing adapted to be incorporated in the diagnostic instrument and including an illumination chamber for receiving a sample carrier;

an array of reflectance light sources mounted within the housing in a fixed position relative to the illumination chamber, and including a first light-emitting diode for emitting substantially monochromatic light of a first wavelength and a second light-emitting diode for emitting substantially monochromatic light of a second wavelength substantially different from the first wavelength;

a light guide mounted in the housing to receive light from each of the light-emitting diodes, for conveying, when only one of the light-emitting diodes is illuminated, substantially all of the light from the one light-emitting diode to the illumination chamber so that the illumination chamber is illuminated substantially uniformly;

a light detector coupled to receive light from the illumination chamber;

a fluorescence excitation light source positioned in the housing and positioned to direct excitation light of a predetermined wavelength to the illumination chamber; and

a light filter positioned between the illumination chamber and the light detector and adapted to prevent passage therethrough of the excitation light from the fluorescence excitation light source but allow passage of emissive light from a sample carrier in the illumination chamber having a wavelength different from the predetermined wavelength of the excitation light.

2. A readhead as defined in claim 1 wherein the fluorescence excitation light source comprises an ultraviolet light source.

3. A readhead as defined in claim 2 wherein the ultraviolet light source comprises a black fluorescent lamp having a line output at 364 nanometers, 405 nanometers, and 436 nanometers, and a broadband output from 330-385 nanometers.

4. A readhead as defined in claim 3 wherein the black fluorescent lamp is positioned in an ultraviolet light source chamber of the housing, and the ultraviolet light source chamber is lined with metal foil.

5. A readhead as defined in claim 1 wherein a mirror is positioned between the light detector and the light filter.

6. A readhead as defined in claim 1 wherein a lens is positioned between the light detector and the light filter and adapted to focus light on the light detector.

7. A diagnostic instrument including a readhead as defined in claim 1 and further comprising a processor connected to the light detector and programmed to process electrical signals from the light detector to determine a physical property of a sample on a sample carrier received in the illumination chamber of the readhead.

8. A diagnostic instrument as defined in claim 7 wherein the processor is programmed to use a predictive model to determine the physical property.

9. A readhead as defined in claim 1 wherein the light guide comprises a diverging light guide having a relatively small width at a point adjacent an inlet of the diverging light guide and a relatively large width at a point adjacent an outlet of the diverging light guide.

20. A readhead as defined in claim 1S wherein the ultraviolet light source is positioned in a chamber of the housing, lined with metal foil.

28. A diagnostic instrument as defined in claim 7 wherein the physical property comprises a glucose concentration.

35. A readhead as defined in claim 2 wherein the ultraviolet light source comprises a light-emitting diode.

36. A readhead as defined in claim 35 wherein the light-emitting diode comprises a high intensity green light-emitting diode.

37. A readhead as defined in claim 35 wherein the ultraviolet light-emitting diode is positioned next to the reflectance light source and the light guide comprises glass or quartz.

10. A readhead for a diagnostic instrument for illuminating a sample carrier and receiving light from the sample carrier, the readhead comprising:

a housing adapted to be incorporated in the diagnostic instrument and adapted to receive and support a sample carrier;

a reflectance light source comprising a light-emitting diode mounted within the housing in a fixed position relative to the sample carrier;

a diverging light guide, mounted in the housing to receive light from the light-emitting diode and adapted to convey substantially all of the light from the light-emitting diode to the sample carrier so that the sample carrier is illuminated substantially uniformly, the diverging light guide having a relatively small width at a point adjacent an inlet of the diverging light guide and a relatively large width at a point adjacent an outlet of the diverging light guide;

a light detector coupled to receive light from the sample carrier;

a fluorescence excitation light source for directing excitation light of a predetermined wavelength to the sample carrier; and

a light filter positioned between the sample carrier and the light detector and adapted to prevent passage therethrough of the excitation light from the fluorescence excitation light source but allow passage of emissive light from the sample carrier having a wavelength different from the predetermined wavelength of the excitation light.

11. A readhead as defined in claim 10 wherein the fluorescence excitation light source comprises an ultraviolet light source.

12. A readhead as defined in claim 11 wherein the ultraviolet light source comprises a black fluorescent lamp having a line output at 364 nanometers, 405 nanometers, and 436 nanometers, and a broadband output from 330-385 nanometers.

13. A readhead as defined in claim 10 wherein a mirror is positioned between the light detector, and the light filter.

14. A readhead as defined in claim 10 wherein a lens is positioned between the light detector and the light filter and adapted to focus light on the light detector.

15. A diagnostic instrument including a readhead as defined in claim 10 and further comprising a processor connected to the light detector and programmed to process electrical signals from the light detector to determine a physical property of a sample on a sample carrier received in the readhead.

16. A diagnostic instrument as defined in claim 15 wherein the processor is programmed to use a predictive model to determine the physical property.

27. A diagnostic instrument as defined in claim 15 wherein the physical property comprises a glucose concentration.

32. A readhead as defined in claim 11 wherein the ultraviolet light source comprises a light-emitting diode.

33. A readhead as defined in claim 32 wherein the light-emitting diode comprises a high intensity green light-emitting diode.

34. A readhead as defined in claim 32 wherein the ultraviolet light-emitting diode is positioned next to the reflectance light source and the light guide comprises glass or quartz.

17. A readhead for a diagnostic instrument for illuminating a sample carrier and receiving light from the sample carrier, the readhead comprising:

a housing adapted to be incorporated in the diagnostic instrument and including an illumination chamber for receiving a sample carrier;

a reflectance light source comprising a lensless light-emitting diode mounted within the housing in a fixed position relative to the illumination chamber;

a light guide, mounted in a fixed position relative to the lensless light-emitting diode, for conveying light from the lensless light-emitting diode to a sample carrier in the illumination chamber;

a light detector coupled to receive light from the sample carrier;

a fluorescence excitation light source positioned in the housing for directing excitation light of a predetermined wavelength to the illumination chamber; and

a light filter positioned between the illumination chamber and the light detector and adapted to prevent passage therethrough of the excitation light from the fluorescence excitation light source but allow passage of emissive light from a sample carrier in the illumination chamber having a wavelength different from the predetermined wavelength of the excitation light.

18. A readhead as defined in claim 17 wherein the fluorescence excitation light source comprises an ultraviolet light source.

19. A readhead as defined in claim 18 wherein the ultraviolet light source comprises a black fluorescent lamp having a line output at 364 nanometers, 405 nanometers, and 436 nanometers, and a broadband output from 330-385 nanometers.

21. A readhead as defined in claim 17 wherein a mirror is positioned between the light detector and the light filter.

22. A readhead as defined in claim 17 wherein a lens is positioned between the light detector and the light filter and adapted to focus light on the light detector.

23. A diagnostic instrument including a readhead as defined in claim 17 and further comprising a processor connected to the light detector and programmed to process electrical signals from the light detector to determine a physical property of a sample on a sample carrier received in the illumination chamber of the readhead.

24. A diagnostic instrument as defined in claim 23 wherein the processor is programmed to use a predictive model to determine the physical property.

25. A readhead as defined in claim 17 wherein the light guide comprises a diverging light guide having a relatively small width at a point adjacent an inlet of the diverging light guide and a relatively large width at a point adjacent an outlet of the diverging light guide.

26. A diagnostic instrument as defined in claim 23 wherein the physical property comprises a glucose concentration.

29. A readhead as defined in claim 18 wherein the ultraviolet light source comprises a light-emitting diode.

30. A readhead as defined in claim 29 wherein the light-emitting diode comprises a high intensity green light-emitting diode.

31. A readhead as defined in claim 29 wherein the ultraviolet light-emitting diode is positioned next to the reflectance light source and the light guide comprises glass or quartz.