Oligonucleotide for genotyping of mycoplasma, microarray comprising the oligonucleotide, and method for detection of species using the microarray
First Claim
1. An oligonucleotide for genus-specific genotyping of Mycoplasma and Ureaplasma strains, comprising any one sequence selected from SEQ ID Nos. 7 to 21 or its complementary sequence.
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Abstract
The present invention relates to a method for detecting Mycoplasma and its related strains which are source of contamination of cell lines and biological products and human pathogenic. More particularly, the present invention relates to genus-specific and species-specific oligonucleotides for genotyping of Mycoplasma, Acholeplasm and Ureaplasma strains, microarray comprising the oligonucleotides, and method for detection of species using the microarray.
As described above, the present invention provides a rapid and accurate assay method capable of simultaneously detecting many Mycoplasma and its related strains from a single sample using a microarray comprising novel oligonucleotides for detecting Mycoplasma and its related strains which are known as a source of contamination of cell lines and biological products and human pathogenic. Further, the present invention provides an objective and credible assay method capable of tracing a contamination source for preventing expansion of infective Mycoplasma and its related strains and controlling a contamination of Mycoplasma against biological products and stem cells or cord blood cells which are useful for gene therapy and cell therapy.
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Citations
9 Claims
- 1. An oligonucleotide for genus-specific genotyping of Mycoplasma and Ureaplasma strains, comprising any one sequence selected from SEQ ID Nos. 7 to 21 or its complementary sequence.
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2. An oligonucleotide for species-specific genotyping of Mycoplasma and Ureaplasma strains, comprising any one sequence selected from SEQ ID Nos. 28 to 127 or its complementary sequence.
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3. An oligonucleotide for genus-specific genotyping of Acholeplasma strains, comprising any one sequence selected from SEQ ID Nos. 22 to 27 or its complementary sequence.
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4. An oligonucleotide for species-specific genotyping of Acholeplasma strains, comprising any one sequence selected from SEQ ID Nos. 128 to 133 or its complementary sequence.
Specification