Biochemical reaction cartridge

• US 20070071637A1
• Filed: 10/13/2006
• Published: 03/29/2007
• Est. Priority Date: 03/31/2003
• Status: Active Grant

• Current Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Original Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Inventors: NUMAJIRI, YASUYUKI
• Granted Patent: US 7,988,913 B2
• Time in Patent Office: Days
• US Class Current: 422/57
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2300/1822 using Peltier elements
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046

Abstract

A biochemical reaction cartridge includes an injection port for injecting a specimen, a chamber for containing the specimen therein, a chamber for containing a reagent for treating the specimen, nozzle ports for applying or reducing pressure by using fluid. In the cartridge, the specimen is subjected to a biochemical reaction by controlling the fluid. The cartridge is mounted in a biochemical reaction apparatus.

Claims

1. A biochemical reaction cartridge, comprising:

a storage chamber for accumulating a liquid,

a first chamber,

a first passage for connecting the storage chamber to the first chamber to move the liquid in the storage chamber to the first chamber,

a second chamber, and

a second passage for connecting the first chamber to the second chamber to move the liquid in the first chamber to the second chamber,

wherein a bottom position of a first connecting portion for connecting the first chamber to the first passage is higher than a bottom position of a second connecting portion for connecting the first chamber to the second passage.

2. The cartridge according to claim 1, wherein the liquid is caused to flow to the first chamber so that the first chamber has a maximum liquid level lower than the bottom position of the first connecting position.

3. The cartridge according to claim 1, wherein movement of the liquid is controlled by externally applying or reducing pressure.

4. The cartridge according to claim 2, wherein movement of the liquid is controlled by externally applying or reducing pressure.

5. The cartridge according to claim 3, wherein the cartridge comprises a pressure reducing portion for externally reducing the pressure, the pressure reducing portion being provided with a chamber for preventing outflow of the liquid.

6. The cartridge according to claim 4, wherein the cartridge comprises a pressure reducing portion for externally reducing the ressure, the pressure reducing portion being provided with a chamber for preventing outflow of the liquid.