Humanized antibodies
1 Assignment
0 Petitions
Accused Products
Abstract
Humanized antibodies that bind ICAM-1 are provided. Antibodies include those selected from: SEQ ID NO:1 and 3 (HumA); SEQ ID NO:5 and 7 (HumB); SEQ ID NO:9 and 11 (HumC); SEQ ID NO:13 and 15 (HumD); SEQ ID NO:17 and 19 (HumE); SEQ ID NO:21 and 23 (HumF); SEQ ID NO:25 and 27 (HumG); SEQ ID NO:29 and 31 (HumH); and SEQ ID NO:33 and 35 (HumI). Subsequences of the humanized antibodies capable of binding an ICAM-1 epitope are also provided. Methods of inhibiting pathogen infection (e.g., HRV) of a cell employing humanized antibodies capable of binding an ICAM-1 epitope are further provided.
97 Citations
62 Claims
-
1-57. -57. (canceled)
-
58. A humanized antibody or subsequence thereof that binds ICAM-1, said antibody comprising i) SEQ ID NO:
- 5 and SEQ ID NO;
7;
ii) a subsequence of SEQ ID NO;
5 and/or SEQ ID NO;
7;
or iii) SEQ ID NO;
5 and SEQ ID NO;
7 wherein an acceptor variable framework region of SEQ ID NO;
5 and/or SEQ ID NO;
7 has at least one non-human donor amino acid.
- 5 and SEQ ID NO;
-
59. A humanized antibody that binds ICAM-1 and is capable of inhibiting human rhinovirus infection of cells expressing ICAM-1, said antibody having VH and VL regions comprising SEQ ID NO:
- 5 and SEQ ID NO;
7 wherein an acceptor variable framework region of SEQ ID NO;
5 and/or SEQ ID NO;
7 has an amino acid substitution in a human consensus variable region sequence, and wherein the protective efficacy against human rhinovirus is greater than a non-humanized antibody containing the variable domains of mouse monoclonal antibody denoted as 1A6.
- 5 and SEQ ID NO;
-
60. A humanized antibody capable of inhibiting human rhinovirus infection of cells comprising VH and VL regions having amino acid sequences comprising i) SEQ ID NO:
- 5 and SEQ ID NO;
7, or ii) SEQ ID NO;
5 and SEQ ID NO;
7 wherein an acceptor variable framework region of one or both of the preceding comprises at least one non-human donor amino acid.
- 5 and SEQ ID NO;
-
61. A antibody or subsequence thereof comprising SEQ ID NO:
- 5 and SEQ ID NO;
7.
- 5 and SEQ ID NO;
-
62. A antibody or subsequence thereof comprising:
-
i) SEQ ID NO;
5 and SEQ ID NO;
7;
orii) SEQ ID NO;
5 and SEQ ID NO;
7 wherein an acceptor variable framework region of one or both of the preceding has at least one non-human donor amino acid, wherein said antibody binds ICAM-1.
-
Specification