Epitopes
First Claim
Patent Images
1. A composition comprising a polypeptide having the amino acid sequence of SEQ ID NO:
- 6.
2 Assignments
0 Petitions
Accused Products
Abstract
Compositions and methods relating to epitopes of sclerostin protein, and sclerostin binding agents, such as antibodies capable of binding to sclerostin, are provided.
154 Citations
66 Claims
- 1. A composition comprising a polypeptide having the amino acid sequence of SEQ ID NO:
-
2. A composition comprising a polypeptide having the amino acid sequence of SEQ ID NO:
- 63.
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3. A composition comprising a polypeptide having the amino acid sequence of SEQ ID NO:
- 64.
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4. A composition comprising a polypeptide having the amino acid sequence of SEQ ID NO:
- 65.
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5. A composition comprising a polypeptide having the amino acid sequence of SEQ ID NO:
- 66.
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6. A composition comprising a polypeptide having the amino acid sequence of SEQ ID NO:
- 67.
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7. A composition comprising a polypeptide having the amino acid sequence of SEQ ID NO:
- 68.
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8. A composition comprising a polypeptide having the amino acid sequence of SEQ ID NO:
- 69.
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9. A composition comprising at least one polypeptide of the amino acid sequence of SEQ ID NO:
- 2, SEQ ID NO;
3, SEQ ID NO;
4 or SEQ ID NO;
5. - View Dependent Claims (10, 11, 12)
- 2, SEQ ID NO;
-
13. A composition comprising a polypeptide having the amino acid sequences of SEQ ID NO:
- 2, SEQ ID NO;
3, SEQ ID NO;
4 and SEQ ID NO;
5, wherein SEQ ID NO;
2 and 4 are joined by a disulfide bond at amino acid positions 57 and 111 with reference to SEQ ID NO;
1, and SEQ ID NO;
3 and 5 are joined by at least one of (a) a disulfide bond at amino acid positions 82 and 142 with reference to SEQ ID NO;
1, and (b) a disulfide bond at amino acid positions 86 and 144 with reference to SEQ ID NO;
1.
- 2, SEQ ID NO;
-
15. A polypeptide consisting essentially of a multiply truncated human sclerostin protein of SEQ ID NO:
- 1, wherein amino acids 1-50, 65-72, 91-100, 118-137, and 150-190 of SEQ ID NO;
1 are absent from said polypeptide.
- 1, wherein amino acids 1-50, 65-72, 91-100, 118-137, and 150-190 of SEQ ID NO;
-
16. A composition comprising at least one polypeptide consisting essentially of the amino acid sequence of SEQ ID NO:
- 70, SEQ ID NO;
71, SEQ ID NO;
72 or SEQ ID NO;
73. - View Dependent Claims (17, 18, 19, 21)
- 70, SEQ ID NO;
-
20. A composition comprising a polypeptide having the amino acid sequences of SEQ ID NO:
- 70, SEQ ID NO;
71, SEQ ID NO;
72 and SEQ ID NO;
73, wherein SEQ ID NO;
72 and 73 are joined by a disulfide bond at amino acid positions 57 and 111 with reference to SEQ ID NO;
1, and SEQ ID NO;
70 and 71 are joined by at least one of (a) a disulfide bond at amino acid positions 82 and 142 with reference to SEQ ID NO;
1, and (b) a disulfide bond at amino acid positions 86 and 144 with reference to SEQ ID NO;
1.
- 70, SEQ ID NO;
-
22. A polypeptide consisting essentially of a multiply truncated human sclerostin protein of SEQ ID NO:
- 1, wherein amino acids 1-56, 65-72, 87-110, 118-137, and 145-190 of SEQ ID NO;
1 are absent from said polypeptide. - View Dependent Claims (23, 24)
- 1, wherein amino acids 1-56, 65-72, 87-110, 118-137, and 145-190 of SEQ ID NO;
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25. A portion of human sclerostin comprising amino acids 51-64, 73-90, 101-117, and 138-149 of SEQ ID NO:
- 1, wherein said portion has at least one of;
(a) a disulfide bond between amino acids 57 and 111;
(b) a disulfide bond between amino acids 82 and 142; and
(c) a disulfide bond between amino acids 86 and 144. - View Dependent Claims (26, 27)
- 1, wherein said portion has at least one of;
-
28. A portion of human sclerostin comprising amino acids 57-64, 73-86, 111-117, and 138-144 of SEQ ID NO:
- 1, wherein said portion has at least one of;
(a) a disulfide bond between amino acids 57 and 111;
(b) a disulfide bond between amino acids 82 and 142; and
(c) a disulfide bond between amino acids 86 and 144. - View Dependent Claims (29, 30)
- 1, wherein said portion has at least one of;
-
31. A polypeptide consisting essentially of a human sclerostin protein of SEQ ID NO:
- 1 truncated at the C-terminal and N-terminal ends, wherein amino acids 1-85 and 112-190 of SEQ ID NO;
1 are absent from said polypeptide.
- 1 truncated at the C-terminal and N-terminal ends, wherein amino acids 1-85 and 112-190 of SEQ ID NO;
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32. A portion of human sclerostin, comprising amino acids 86-111 of SEQ ID NO:
- 1.
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33. A portion of human sclerostin, comprising SEQ ID NO:
- 6.
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34. A portion of human sclerostin, comprising SEQ ID NO:
- 63.
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35. A portion of human sclerostin, comprising SEQ ID NO:
- 64.
-
36. A portion of human sclerostin, comprising SEQ ID NO:
- 65.
-
37. A portion of human sclerostin, comprising SEQ ID NO:
- 66.
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38. A portion of human sclerostin, comprising SEQ ID NO:
- 67.
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39. A portion of human sclerostin, comprising SEQ ID NO:
- 68.
-
40. A portion of human sclerostin, comprising SEQ ID NO:
- 69.
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41. A portion of rat sclerostin, comprising SEQ ID NO:
- 96.
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42. A portion of rat sclerostin, comprising SEQ ID NO:
- 97.
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43. A method of producing a portion of human sclerostin, comprising the steps of:
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(a) treating human sclerostin with trypsin to achieve complete tryptic digestion;
(b) collecting the tryptic digest sample having average molecular mass of 7122.0D (theoretical mass 7121.5D) or retention time of about 20.6 minutes as determined by elution from a reverse-phase HPLC column with linear gradient from 0.05% trifluoroacetic acid to 90% acetonitrile in 0.05% TFA at a flow rate of 0.2 ml/min; and
(c) purifying said portion.
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44. A polypeptide of SEQ ID NO:
- 6.
-
45. A polypeptide of SEQ ID NO:
- 2.
-
46. A polypeptide of SEQ ID NO:
- 3.
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47. A polypeptide of SEQ ID NO:
- 4.
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48. A polypeptide of SEQ ID NO:
- 5.
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49. A polypeptide of SEQ ID NO:
- 2, SEQ ID NO;
3, SEQ ID NO;
4 and SEQ ID NO;
5.
- 2, SEQ ID NO;
-
50. A polypeptide of SEQ ID NO:
- 96.
-
51. A polypeptide of SEQ ID NO:
- 97.
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52. A polypeptide of SEQ ID NO:
- 63.
-
53. A polypeptide of SEQ ID NO:
- 64
-
54. A polypeptide of SEQ ID NO:
- 65.
-
55. A polypeptide of SEQ ID NO:
- 66.
-
56. A polypeptide of SEQ ID NO:
- 67.
-
57. A polypeptide of SEQ ID NO:
- 68.
-
58. A polypeptide of SEQ ID NO:
- 69.
-
59. A polypeptide of SEQ ID NO:
- 70.
-
60. A polypeptide of SEQ ID NO:
- 71.
-
61. A polypeptide of SEQ ID NO:
- 72.
-
62. A polypeptide of SEQ ID NO:
- 73.
-
63. A polypeptide of SEQ ID NO:
- 70, SEQ ID NO;
71, SEQ ID NO;
72 and SEQ ID NO;
73.
- 70, SEQ ID NO;
-
66. A vaccine comprising at least one polypeptide comprising one or more of the epitopes of SEQ ID NO:
- 2, 3, 4, 5, 6, 70, 71, 72, and 73.
Specification