Specimen analysis system obtaining characteristic of specimen by diffusion approximation

• US 20070073157A1
• Filed: 09/20/2006
• Published: 03/29/2007
• Est. Priority Date: 09/20/2005
• Status: Active Grant

• Current Assignee: Fujifilm Corporation (Fujifilm Holdings Corporation)
• Original Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Inventors: Sendai, Tomonari
• Granted Patent: US 7,477,393 B2
• Time in Patent Office: Days
• US Class Current: 600/473
• CPC Class Codes: A61B 5/0073 by tomography, i.e. reconstruction of 3D images from 2D projections A61B5/0066 takes precedence
• CPC Class Codes: G01N 21/49 within a body or fluid
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53

Abstract

In a specimen analysis system: a light injection unit selectively injects unscattered light and scattered light into a specimen at an incident position on the specimen, and an information processing unit performs calculation of a characteristic of the specimen by diffusion approximation, where the calculation of the characteristic in a near-incident-point region located within a predetermined distance from the incident position is based on information carried by light which exits from the near-incident-point region of the specimen in response to injection of the scattered light into the specimen, and the calculation of the characteristic in a remote region located farther from the incident position than the near-incident-point region is based on information carried by light which exits from the remote region in response to injection of the unscattered light into the specimen.

Claims

1. A specimen analysis system comprising:

a light injection unit which selectively injects unscattered light and scattered light into a specimen at an incident position on the specimen, where the scattered light is generated by scattering by a scattering medium;

an information acquisition unit which acquires first information carried by first light exiting from a plurality of positions in a first region of said specimen in response to injection of said scattered light into the specimen, and second information carried by second light exiting from a plurality of positions in a second region of the specimen in response to injection of said unscattered light into the specimen, where the first region is located within a predetermined distance from said incident position, and the second region is located farther from the incident position than the first region; and

an information processing unit which calculates a characteristic of said specimen by diffusion approximation, where calculation of the characteristic in said first region is based on said first information and calculation of the characteristic in said second region is based on said second information.

2. A specimen analysis system according to claim 1, wherein said light injection unit alternately applies said unscattered light and said scattered light to said specimen.

3. A specimen analysis system according to claim 1, wherein said information acquisition unit further acquires third information carried by third light exiting from a plurality of positions in a third region of said specimen in response to injection of said unscattered light into the specimen, and fourth information carried by fourth light exiting from a plurality of positions in the third region of the specimen in response to injection of said scattered light into the specimen, where the third region is located farther from the incident position than the first region and nearer to the incident position than the second region, and said information processing unit compares the third information and the fourth information, and calculates the characteristic in said third region on the basis of said third information when a difference between the third information and the fourth information is equal to or smaller than a predetermined amount, and on the basis of said fourth information when the difference between the third information and the fourth information is greater than the predetermined amount.

4. A specimen analysis system according to claim 2, wherein said information acquisition unit further acquires third information carried by third light exiting from a plurality of positions in a third region of said specimen in response to injection of said unscattered light into the specimen, and fourth information carried by fourth light exiting from a plurality of positions in the third region of the specimen in response to injection of said scattered light into the specimen, where the third region is located farther from the incident position than the first region and nearer to the incident position than the second region, and said information processing unit compares the third information and the fourth information, and calculates the characteristic in said third region on the basis of said third information when a difference between the third information and the fourth information is equal to or smaller than a predetermined amount, and on the basis of said fourth information when the difference between the third information and the fourth information is greater than the predetermined amount.

5. A specimen analysis system according to claim 1, wherein said first light and said second light include fluorescence emitted from said specimen.

6. A specimen analysis system according to claim 2, wherein said first light and said second light include fluorescence emitted from said specimen.

7. A specimen analysis system according to claim 3, wherein said first light, said second light, said third light, and said fourth light include fluorescence emitted from said specimen.

8. A specimen analysis system according to claim 4, wherein said first light, said second light, said third light, and said fourth light include fluorescence emitted from said specimen.

9. A specimen analysis system comprising:

a light injection unit which contains a scattering medium scattering unscattered light to a degree of scattering which can be controlled stepwise or continuously, produces first light and second light by scattering the unscattered light with the scattering medium to first and second degrees of scattering, respectively, and selectively injects the first light and the second light into a specimen at an incident position on the specimen, where the first degree of scattering is higher than the second degree of scattering;

a scattering control unit which controls the degree of scattering in the scattering medium;

an information acquisition unit which acquires first information carried by third light exiting from a plurality of positions in a first region of said specimen in response to injection of said first light into the specimen, and second information carried by fourth light exiting from a plurality of positions in a second region of the specimen in response to injection of said second light into the specimen, where the first region is located within a predetermined distance from said incident position, and the second region is located farther from the incident position than the first region; and

an information processing unit which calculates a characteristic of said specimen by diffusion approximation, where calculation of the characteristic in said first region is based on said first information, and calculation of the characteristic in said second region is based on said second information.

10. A specimen analysis system according to claim 9, wherein said information processing unit performs a comparison between said first information and said second information, and controls the degree of scattering in the scattering medium according to a result of the comparison.

11. A specimen analysis system according to claim 9, wherein said third light and said fourth light include fluorescence emitted from said specimen.

12. A specimen analysis system according to claim 10, wherein said third light and said fourth light include fluorescence emitted from said specimen.