Apatite particle, method of producing the same, apatite particle-gene complex, and method of gene transfection

• US 20070077306A1
• Filed: 12/27/2004
• Published: 04/05/2007
• Est. Priority Date: 12/26/2003
• Status: Abandoned Application

• Current Assignee: Tokyo Institute of Technology (National University Corporation Tokyo Institute of Technology)
• Original Assignee: Tokyo Institute of Technology (National University Corporation Tokyo Institute of Technology)
• Inventors: Akaike, Toshihiro, Chowdhury, Ezharul
• Application Number:
  US10/584,722
• Publication Number:
  US 20070077306A1
• Time in Patent Office: Days
• US Class Current: 424/489
• CPC Class Codes: C01B 25/32 Phosphates of magnesium, calcium, strontium, or barium
• CPC Class Codes: C12N 15/87 Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Abstract

When a solution of an inorganic phosphoric acid, added to with calcium ions and magnesium ions, is incubated for a preset time duration, an apatite particle obtained is reduced in size, depending on the concentration of the magnesium ions. By employing an apatite particle, for which the concentration of the magnesium ions and the incubation time duration are controlled adequately, it is possible to improve the gene transfection efficiency into the cells and the gene expression efficiency in the cells.

Claims

1-4. -4. (canceled)

5. An apatite particle represented by a molecular formula Ca_10-xMg_x(PO₄)₆(OH)₂, where x=1, 2, . . . , 9, or a chemical formula Ca_8-xMg_xH₂(PO₄)₆, where x=1, 2, . . . , 7, wherein the particle has a size of approximately 30 nm to 2500 nm.

8. A method of producing an apatite particle represented by a molecular formula Ca_10-xMg_x(PO₄)₆(OH)₂, where x=1, 2, . . . , 9, or a chemical formula Ca_8-xMg_xH₂(PO₄)₆, where x=1, 2, . . . , 7, wherein the particle has a size of approximately 30 nm to 2500 nm, the method comprising incubating a solution containing inorganic phosphoric acid, calcium ions and magnesium ions for a predetermined time.

9. The method of claim 8, wherein the particle size is approximately 50 nm to 1000 nm.

10. The method of claim 8, wherein the particle size is approximately 50 nm to 300 nm.

11. An apatite particle- gene complex in which a specified gene is combined with an apatite particle, the particle being represented by a molecular formula Ca_10-xMg_x(PO₄)₆(OH)₂, where x=1, 2, . . . 9, or a chemical formula Ca_8-xMg_xH₂(PO₄)₆, where x=1, 2, . . . , 7, wherein the particle has a size of approximately 30 nm to 2500 nm.

12. The apatite particle-gene complex of claim 11, wherein the particle size is approximately 50 nm to 1000 nm.

13. The apatite particle-gene complex of claim 11, wherein the particle size is approximately 50 nm to 300 nm.

14. A method of transfecting a preset gene into a specified cell by incubating, with said specified cell, an apatite particle- gene complex in which a preset gene is combined with an apatite particle represented by a molecular formula Ca_10-xMg_x(PO₄)₆(OH)₂, where x=1, 2, . . . , 9, or by a chemical formula Ca_8-xMg_xH₂(PO₄)₆, where x=1, 2, . . . , 7, wherein the particle has a size of approximately 30 nm to 2500 nm.

15. The method of claim 14, wherein the particle size is approximately 50 nm to 1000 nm.

16. The method of claim 14, wherein the particle size is approximately 50 nm to 300 nm.