Compositions and methods for in vitro sorting of molecular and cellular libraries
First Claim
1. A library comprising a plurality of distinct molecules encapsulated within a water-in-oil-in-water emulsion, the emulsion comprising a continuous external aqueous phase and a discontinuous dispersion of water-in-oil droplets, wherein the internal aqueous phase of a plurality of the droplets each comprises a specific molecule of said plurality of distinct molecules of the library.
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Abstract
The present invention provides an in vitro system for compartmentalization of molecular or cellular libraries and provides methods for selection and isolation of desired molecules or cells from the libraries. The library includes a plurality of distinct molecules or cells encapsulated within a water-in-oil-in-water emulsion. The emulsion includes a continuous external aqueous phase and a discontinuous dispersion of water-in-oil droplets. The internal aqueous phase of a plurality of such droplets comprises a specific molecule or cell that is within the plurality of distinct molecules or cells of the library.
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Citations
26 Claims
- 1. A library comprising a plurality of distinct molecules encapsulated within a water-in-oil-in-water emulsion, the emulsion comprising a continuous external aqueous phase and a discontinuous dispersion of water-in-oil droplets, wherein the internal aqueous phase of a plurality of the droplets each comprises a specific molecule of said plurality of distinct molecules of the library.
Specification