In vitro sorting method
First Claim
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1. A method of identifying an expressible genetic element, comprising:
- a) expressing a genetic element of a plurality of genetic elements to produce an expressed product, wherein said plurality of said genetic elements are dispersed in aqueous droplets of an emulsion; and
b) separating a said aqueous droplet that contains said expressed product from those aqueous droplets that do not, thereby identifying said expressed genetic element.
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Abstract
The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising of the steps of: (a) compartmentalising genetic elements into microcapsules; (b) expressing the genetic elements to produce their respective gene products within the microcapsules; (c) sorting the genetic elements which produce the gene product having a desired activity. The invention enables the in vitro evolution of nucleic acids by repeated mutagenesis and iterative applications of the method of the invention.
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Citations
33 Claims
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1. A method of identifying an expressible genetic element, comprising:
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a) expressing a genetic element of a plurality of genetic elements to produce an expressed product, wherein said plurality of said genetic elements are dispersed in aqueous droplets of an emulsion; and
b) separating a said aqueous droplet that contains said expressed product from those aqueous droplets that do not, thereby identifying said expressed genetic element. - View Dependent Claims (33)
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2-24. -24. (canceled)
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25. A method of identifying an expressible genetic element, comprising:
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a) expressing a genetic element of a plurality of genetic elements to produce an expressed product, wherein said plurality of said genetic elements are dispersed in aqueous droplets of an emulsion; and
b) physically associating the expressed product of step (a) with the genetic element that encodes it; and
c) separating the genetic element that is physically associated with its expressed product, resulting from step (b), from genetic elements that are not associated with said expressed product, thereby identifying an expressible genetic element.
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26-31. -31. (canceled)
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32. A method of preparing a gene product, said method comprising:
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a) expressing a genetic element of a plurality of genetic elements to produce an expressed product, wherein said plurality of said genetic elements are dispersed in aqueous droplets of an emulsion;
b) separating an aqueous droplet that contains said expressed product from those that do not; and
c) expressing the product of said genetic element, thereby producing said gene product.
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Specification