Apparatus and methods for evaluating an optical property of a liquid sample

• US 20070081159A1
• Filed: 10/11/2005
• Published: 04/12/2007
• Est. Priority Date: 10/11/2005
• Status: Abandoned Application

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Kralik, John, Wilson, William, Giffin, Kristin
• Application Number:
  US11/247,355
• Publication Number:
  US 20070081159A1
• Time in Patent Office: Days
• US Class Current: 356/319
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0838 Capillaries
• CPC Class Codes: B01L 2300/0858 Side walls
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 3/0275 Interchangeable or disposable dispensing tips
• CPC Class Codes: B01L 3/508 rigid containers not provided for above
• CPC Class Codes: G01N 2021/0382 Frustoconical, tapered cell
• CPC Class Codes: G01N 2021/6417 Spectrofluorimetric devices
• CPC Class Codes: G01N 2035/1039 Micropipettes, e.g. microcapillary tubes
• CPC Class Codes: G01N 21/03 Cuvette constructions
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/11 Filling or emptying of cuvettes
• CPC Class Codes: G01N 21/15 Preventing contamination of the components of the optical system or obstruction of the light path
• CPC Class Codes: G01N 21/33 using ultraviolet light G01N21/39 takes precedence

Abstract

An apparatus for acquiring and holding liquid samples and methods using the same.

Claims

1. An apparatus for holding a liquid sample, the apparatus comprising:

a body comprising;

a first opening located at a first end;

a second opening located at a second end;

an inner space of said body connecting said first opening and said second opening, providing a passage from said first opening to said second opening;

at least a portion of at least one inner surface of said passage and a corresponding outer surface of said body being at least partially transparent to electromagnetic radiation;

at least a portion of said passage comprising a measurement region with a predetermined optical pathlength; and

said passage being dimensioned to preclude holding the liquid sample in said measurement region by capillary action.

2. The apparatus of claim 1 further comprising:

a hollow adaptor body comprising;

a first opening at a first adaptor body end, and

a second opening at a second adaptor body end,

said second opening of said adaptor body being capable of providing a substantially gastight connection to said first end of said body,

said first adaptor body end and said first opening at said first adaptor body end being capable of operatively connecting to a device for aspirating fluid.

3. The apparatus of claim 1 wherein said first opening is capable of being operatively connected to a device for aspirating fluid.

4. The apparatus of claim 1 wherein said body comprises at least two sections;

wherein one of said at least two sections comprises said first opening and said first end; and

,

wherein said first end is capable of operatively connecting to a device for aspirating fluid.

5. The apparatus of claim 4 wherein said at least two sections comprise at least three sections;

and

at least one other of said at least three sections comprises two planar inner surfaces and two planar outer surfaces.

6. The apparatus of claim 1 further comprising:

an outer coating layer disposed over a portion of an outer surface of said body.

7. The apparatus of claim 6 wherein at least a portion of said outer surfaces is not covered by said outer coating layer.

8. The apparatus of claim 7 wherein an outer surface of said body is at least partially coated with or comprises a hydrophobic material.

9. The apparatus of claim 1 further comprising:

a housing receiving said body;

said housing comprising;

a first housing opening, and a second housing opening,

said first housing opening and said second housing opening being substantially aligned;

said first housing opening, said second housing opening and said measurement region of said body constituting a transmission path for electromagnetic radiation when said body is held in said housing.

10. The apparatus of claim 9 wherein said first housing opening and/or said second housing opening are capable of being in optical communication with an optical fiber.

11. The apparatus of claim 9 further comprising:

a third housing opening, and a fourth housing opening,

said third housing opening and said fourth housing opening being substantially aligned;

said third housing opening, said fourth housing opening and said measurement region of said body constituting another transmission path for electromagnetic radiation when said body is held in said-housing.

12. The apparatus of claim 11 wherein said third housing opening and/or said fourth housing opening are capable of being in optical communication with an optical fiber.

13. The apparatus of claim 9 further comprising:

a securing component interior of said housing, said securing component being capable of securing said body in at least one predetermined position.

14. The apparatus of claim 13 wherein the first housing opening is capable of being connected to an end of a source-side optical fiber and the second housing opening is capable of being connected to detection-side optical fiber of an optical detection device.

15. The apparatus of claim 13 wherein said at least one predetermined position comprises a plurality of positions.

16. The apparatus of claim 1 wherein said passage comprises a first section and a second section and wherein the dimensions of a cross-section through said first and second section are different.

17. A measurement system capable of measuring a liquid sample, the measurement system comprising:

a source of electromagnetic radiation;

an optical component capable of providing at least two beams of electromagnetic radiation from electromagnetic radiation originating from said source;

a component capable of holding an apparatus in a path of each of said at least two beams, said apparatus capable of holding a liquid sample and comprising;

a body comprising;

a first opening located at a first end;

a second opening located at a second end;

an inner space of said body connecting said first opening and said second opening, providing a passage from said first opening to said second opening;

at least a portion of at least one inner surface of said inner space and a corresponding outer surface of said body being at least partially transparent;

and at least a portion of said passage comprising a measurement region with a predetermined optical pathlength; and

at least two optical delivery components, each optical delivery component disposed to receive one of said at least two beams after propagating through said apparatus; and

a detecting component optically disposed to receive at least one of said at least two beams from at least one of said at least two optical delivery components and capable of obtaining a measurement therefrom.

18. The measurement system of claim 17 wherein said at least two optical delivery components comprise at least two reflecting components, each one of said at least two reflecting components being capable of deflecting one of said at least two beams of electromagnetic radiation.

19. The measurement system of claim 18 further comprising:

a beam selecting component capable of selecting one beam from said at least two beams of electromagnetic radiation, after said at least two beams are deflected;

said beam selecting component being optically disposed to receive said two beams of electromagnetic radiation after being deflected.

20. The measurement system of claim 17 wherein said passage, in said apparatus, comprises a first section and a second section and wherein a cross-section of said first section is different from a cross-section of said second section.

21. The measurement system of claim 17 wherein a dimension of at least a portion of said passage, in said apparatus, varies substantially monotonically from one end of said at least a portion to another end of said at least a portion.

22. A method for measuring an optical property of a liquid sample, comprising the steps of:

providing an apparatus having a body comprising;

a first opening located at a first end;

a second opening located at a second end;

an inner space of said body connecting said first opening and said second opening, providing a passage from said first opening to said second opening;

at least a portion of at least one inner surface of said inner space and a corresponding outer surface of said body being at least partially transparent and forming a measurement region with a predetermined optical pathlength;

placing the measurement region of the apparatus in a positional relationship to a light source and detector of an optical detection device such that a light path is provided from the light source through the measurement region, to the optical detection device, wherein the measurement region of the apparatus holds the liquid sample.

23. The method of claim 22 further comprising the steps of:

interfacing the apparatus with a device for aspirating fluid; and

aspirating the liquid sample into the measurement area.

24. The method of claim 22, wherein the optical detection device is a spectrophotometer.