CULTURE CELLS FROM PANCREATIC ISLETS

• US 20070081980A1
• Filed: 12/08/2006
• Published: 04/12/2007
• Est. Priority Date: 12/04/2001
• Status: Abandoned Application

• Current Assignee: Organogenesis Inc. (Organogenesis Holdings, Inc.)
• Original Assignee: Organogenesis Inc. (Organogenesis Holdings, Inc.)
• Inventors: Jin, Jianjian
• Application Number:
  US11/608,542
• Publication Number:
  US 20070081980A1
• Time in Patent Office: Days
• US Class Current: 424/93.700
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61P 3/10 for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: C12N 2500/25 Insulin-transferrin; Insulin-transferrin-selenium
• CPC Class Codes: C12N 2500/32 Amino acids
• CPC Class Codes: C12N 2500/34 Sugars
• CPC Class Codes: C12N 2500/40 Nucleotides, nucleosides, bases cyclic nucleotides C12N2501/01, anti-neoplasic drugs C12N2501/06
• CPC Class Codes: C12N 2501/11 Epidermal growth factor [EGF]
• CPC Class Codes: C12N 2501/39 Steroid hormones
• CPC Class Codes: C12N 2501/395 Thyroid hormones
• CPC Class Codes: C12N 2506/22 from pancreatic cells
• CPC Class Codes: C12N 5/0678 Stem cells; Progenitor cells; Precursor cells

Abstract

A cell composition of endocrine progenitor cells derived from mammalian pancreatic islet cells that can be trans-planted planted into a diabetic patient such t the cells of the cell composition differentiates into functioning insulin-producing beta cells.

Claims

1-3. -3. (canceled)

4. A method for implanting cells functioning as insulin-producing beta cells into a patient with diabetes, comprising:

(a) serially culturing cells derived from human mammalian pancreas islet cells in a defined cultured medium to expand the cells and to produce endocrine precursor cells in the absence of fibroblast overgrowth, wherein the defined culture medium comprises amino acids, growth factors, hormones, and lipids in the absence of serum or organ extracts, and wherein the endocrine precursor cells have less differentiation than the initially cultured cells and wherein the endocrine precursor cells express markers pdx1 and nestin; and

(b) implanting the endocrine precursor cells into a recipient patient with diabetes.