Methods of purifying anti a beta antibodies
First Claim
1. A method for purifying an Aβ
- binding protein having an Fc region from a source liquid comprising the protein and one or more impurities, comprising the steps of;
adsorbing the Aβ
binding protein to an Fc binding agent;
washing the Fc binding agent with a buffer solution containing a divalent cation salt to remove one or more impurities; and
recovering the protein from the Fc binding agent in an elution solution.
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Abstract
The present application provides methods of purifying Aβ binding proteins having a Fc region, for example, anti-Aβ antibodies or antibody fusions, by adsorbing the Aβ binding protein to a Fc binding agent, such as, for example, Protein A or Protein G, followed by a wash with a divalent cation salt buffer to remove impurities and subsequent recovery of the adsorbed Aβ binding protein. The present application also features methods of eluting the purified Aβ binding protein as well as the incorporation of the methods within a purification train. Kits comprising components for carrying out the methods and instructions for use are also provided.
137 Citations
45 Claims
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1. A method for purifying an Aβ
- binding protein having an Fc region from a source liquid comprising the protein and one or more impurities, comprising the steps of;
adsorbing the Aβ
binding protein to an Fc binding agent;
washing the Fc binding agent with a buffer solution containing a divalent cation salt to remove one or more impurities; and
recovering the protein from the Fc binding agent in an elution solution. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44)
- binding protein having an Fc region from a source liquid comprising the protein and one or more impurities, comprising the steps of;
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45. A kit comprising reagents for purifying an Aβ
- binding protein having an Fc region from impurities, the kit comprising,
a reagent selected from the group consisting of;
a divalent cation salt, anda Fc binding agent, and instructions for use.
- binding protein having an Fc region from impurities, the kit comprising,
Specification