Polymer coated nanofibrillar structures and methods for cell maintenance and differentiation
First Claim
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1. A cell culture article comprising:
- (a) a nanofibrillar structure comprising nanofibers, and (b) a coating formed on at least a portion of the nanofibers, the coating comprising a polymer comprising (i) at least one pendent amine-containing group and (ii) at least one pendent latent reacted group that bonds the polymer to the nanofibers.
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Abstract
The invention provides cell-adherent polymeric coatings for articles having a nanofibrillar structure. The coatings include a synthetic, non-biodegradable polymer having at least one pendent amine group, wherein the polymer is covalently immobilized on the article via latent reactive groups. The invention also provides methods for the long term culturing of cells using the polymer coated nanofibrillar structures. The polymer coated nanofibrillar structures of the invention have been found to be particularly useful for the growth and differentiation of cells, including neural precursors.
72 Citations
54 Claims
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1. A cell culture article comprising:
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(a) a nanofibrillar structure comprising nanofibers, and (b) a coating formed on at least a portion of the nanofibers, the coating comprising a polymer comprising (i) at least one pendent amine-containing group and (ii) at least one pendent latent reacted group that bonds the polymer to the nanofibers. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
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34. A method for preparing a cell culture article comprising the steps of:
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(a) obtaining a nanofibrillar structure comprising nanofibers, wherein a coating is formed on at least a portion of the nanofibers, the coating comprising a polymer having (i) at least one pendent amine-containing group and (ii) at least one pendent latent reacted group that bonds the polymer to the nanofibers; and
(b) providing the nanofibrillar structure onto a cell culture surface of a cell culture vessel.
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35. A method for preparing a cell culture article comprising the steps of:
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(a) obtaining a nanofibrillar structure comprising nanofibers;
(b) disposing a coating composition on at least a portion of the nanofibers, the coating composition comprising a polymer having (i) at least one pendent amine-containing group and (ii) at least one pendent latent reactive group; and
(c) treating the coating composition to activate the pendent latent reactive group and bond the polymer to the nanofibers via a latent reacted group, thereby forming a coating on the nanofibers. - View Dependent Claims (36, 37, 38, 39)
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- 40. A method for culturing one or more cells comprising placing the one or more cells in contact with a nanofibrillar structure comprising nanofibers, wherein the nanofibers comprise a coating formed on at least a portion of the nanofibers, the coating comprising a polymer having (i) at least one pendent amine-containing group and (ii) at least one pendent latent reacted group that bonds the polymer to the nanofibers.
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54. A method of producing oligodendrocytes, astrocytes, or neurons, comprising placing a cell selected from the group consisting of precursors of oligodendrocytes, astrocytes, and neurons in contact with a nanofibrillar structure comprising a coating formed on at least a portion of the nanofibrillar structure, the coating comprising a polymer having (i) at least one pendent amine-containing group and (ii) at least one pendent latent reacted group that bonds the polymer to the substrate, wherein the precursors are placed under conditions and for a period of time sufficient for the precursors to develop into the respective oligodendrocytes, astrocytes, or neurons.
Specification