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Compositions and method for stable isotope labelling of biological compounds

  • US 20070082399A1
  • Filed: 07/12/2004
  • Published: 04/12/2007
  • Est. Priority Date: 07/11/2003
  • Status: Abandoned Application
First Claim
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1. A method for producing a nutrient medium for growing mammalian or insect cells in culture whereby for at least one of H, C or N, substantially all atoms in substrates that are used by the cells for synthesis of biomolecules in the nutrient medium are isotopically labelled, whereby the method comprising the steps of:

  • (a) growing an organism on a mineral medium which supports growth of the organism, whereby in the medium substantially all of the assimilable atoms, for at least one of H, C or N, are isotopically labelled, to produce labelled biomass;

    (b) autolysing the biomass of an organism grown as in (a) to produce an autolysate; and

    , (c) composing the nutrient medium by combining the autolysate as obtained in (b) with further components necessary for growth of the mammalian or insect cells.

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