Highly parallel template-based dna synthesizer
First Claim
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1. A method for preparing a plurality of different synthetic nucleic acids, comprising the steps:
- (a) provision of a support with a surface which comprises a plurality of positions at each of which different nucleic acid fragments are present, comprising base sequences which are complementary to the nucleic acids to be prepared, (b) addition of nucleotide building blocks and of an enzyme which brings about generation of different nucleic acids from the complementary base sequences from (a), and (c) detachment of the nucleic acids generated in step (b) and, where appropriate, provision for further operations.
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Abstract
The invention relates to a template-based method for preparing nucleic acid double strands.
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Citations
31 Claims
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1. A method for preparing a plurality of different synthetic nucleic acids, comprising the steps:
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(a) provision of a support with a surface which comprises a plurality of positions at each of which different nucleic acid fragments are present, comprising base sequences which are complementary to the nucleic acids to be prepared, (b) addition of nucleotide building blocks and of an enzyme which brings about generation of different nucleic acids from the complementary base sequences from (a), and (c) detachment of the nucleic acids generated in step (b) and, where appropriate, provision for further operations. - View Dependent Claims (3, 4, 5, 6, 7, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31)
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2. A method for preparing a nucleic acid double strand, comprising the steps:
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(a) provision of a support with a surface which comprises a plurality of positions at each of which different nucleic acid fragments are present, comprising base sequences which are complementary to partial sequences of the nucleic acid double strand to be prepared, (b) addition of nucleotide building blocks and of an enzyme which brings about generation of partial sequences of the nucleic acid double strand to be prepared from the complementary base sequences from (a), and (c) assembly of the partial sequences generated in step b) to give the desired nucleic acid strand. - View Dependent Claims (8)
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Specification