MICROFABRICATED STRUCTURES FOR FACILITATING FLUID INTRODUCTION INTO MICROFLUIDIC DEVICES

• US 20070092400A1
• Filed: 12/29/2006
• Published: 04/26/2007
• Est. Priority Date: 06/06/1997
• Status: Active Grant

• Current Assignee: Calvin Chow, J Parce, Steven Sundberg
• Original Assignee: Calvin Chow, J Parce, Steven Sundberg
• Inventors: Chow, Calvin, Sundberg, Steven, Parce, J.
• Granted Patent: US 7,364,705 B2
• Time in Patent Office: Days
• US Class Current: 422/503
• CPC Class Codes: B01J 19/0093 Microreactors, e.g. miniaturised or microfabricated reactors laboratory containers with capillary fluid transport in microfabricated channels or chambers B01L3/5027
• CPC Class Codes: B01J 2219/00783 Laminate assemblies, i.e. the reactor comprising a stack of plates
• CPC Class Codes: B01J 2219/00826 Quartz
• CPC Class Codes: B01J 2219/00831 Glass
• CPC Class Codes: B01J 2219/00833 Plastic
• CPC Class Codes: B01J 2219/00853 Employing electrode arrangements
• CPC Class Codes: B01J 2219/00891 Feeding or evacuation
• CPC Class Codes: B01J 2219/00909 using filters
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0418 electro-osmotic flow [EOF]
• CPC Class Codes: B01L 2400/0421 electrophoretic flow
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/0688 surface tension valves, capillary stop, capillary break
• CPC Class Codes: B01L 3/0244 using pins
• CPC Class Codes: B01L 3/0262 using touch-off at substrate or container
• CPC Class Codes: B01L 3/5025 : for parallel transport of multiple samples
• CPC Class Codes: B01L 3/50255 : Multi-well filtration
• CPC Class Codes: B01L 3/5027 : by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502715 : characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502723 : characterised by venting arrangements
• CPC Class Codes: B01L 3/50273 : characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502753 : characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation separation in general B01D; microapparatus for analysis using electrophoresis G01N27/44791; sample preparation G01N1/28
• CPC Class Codes: G01N 2035/00237 : Handling microquantities of analyte, e.g. microvalves, capillary networks
• CPC Class Codes: G01N 2035/1037 : Using surface tension, e.g. pins or wires
• CPC Class Codes: G01N 27/44743 : Introducing samples
• CPC Class Codes: G01N 27/44791 : Microapparatus sample containers with integrated microfluidic structures B01L3/5027
• CPC Class Codes: Y10T 436/25125 : Digestion or removing interfering materials
• CPC Class Codes: Y10T 436/25375 : Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/2575 : Volumetric liquid transfer

Abstract

Fluid introduction is facilitated through the use of a port which extends entirely through a microfluidic substrate. Capillary forces can be used to retain the fluid within the port, and a series of samples or other fluids may be introduced through a single port by sequentially blowing the fluid out through the substrate and replacing the removed fluid with an alternate fluid, or by displacing the fluid in part with additional fluid. In another aspect, microfluidic substrates have channels which varying in cross-sectional dimension so that capillary action spreads a fluid only within a limited portion of the channel network. In yet another aspect, the introduction ports may include a multiplicity of very small channels leading from the port to a fluid channel, so as to filter out particles or other contaminants which might otherwise block the channel at the junction between the channel and the introduction port.

Claims

1. A fluid analysis system comprising:

a substrate having a first surface and a second surface; and

a plurality of through-hole ports extending through said substrate, each of said plurality of through-hole ports having a first open end at said first surface, a second open end at said second surface, and a non-uniform cross-section extending therebetween, each of said plurality of through-hole ports being sized to retain said test fluid therein.

2. The fluid analysis system according to claim 1 wherein each of said plurality of through-hole ports defines a tapered cross-section from said first open end to said second open end.

3. The fluid analysis system according to claim 1 wherein each of said plurality of through-hole ports defines a first cross-section adjacent said first open end, a second cross-section adjacent said second open end, and a third cross-section at a position between said first open end and said second open end, said third cross-section being greater than at least one of said first cross-section and said second cross-section.

4. The fluid analysis system according to claim 1 wherein each of said plurality of through-hole ports defines a first cross-section adjacent said first open end, a second cross-section adjacent said second open end, and a third cross-section at a position between said first open end and said second open end, said third cross-section being less than at least one of said first cross-section and said second cross-section.

5. The fluid analysis system according to claim 1 wherein said substrate comprises:

a first portion; and

a second portion coupled with said first portion,

wherein at least one of said plurality of through-hole ports extending through said first portion and said second portion.

6. The fluid analysis system according to claim 5 wherein said at least one of said plurality of through-hole ports comprises a first cross-section within said first portion of said substrate and a second cross-section within said second portion of said substrate, said first cross-section being different than said second cross-section.

7. The fluid analysis system according to claim 1 wherein each of said plurality of through-hole ports defines a circular cross-section having a varying diameter.

8. The fluid analysis system according to claim 1 wherein each of said plurality of through-hole ports defines a rectangular cross-section having a varying cross-sectional dimension.

9. The fluid analysis system according to claim 1 wherein each of said plurality of through-hole ports defines a total volume between said first surface and said second surface of less than 20 μ

L.

10. The fluid analysis system according to claim 1 wherein each of said plurality of through-hole ports defines a total volume between said first surface and said second surface between about 0.5 μ

L and 10 μ

L.

11. The fluid analysis system according to claim 1 wherein a first of said plurality of through-hole ports receives a first test fluid and a second of said plurality of through-hole ports receives a second test fluid, said second test fluid being different than said first test fluid.

12. The fluid analysis system according to claim 1, further comprising:

a plurality of fluid transport members generally aligned with at least some of said plurality of through-hole ports to transport said test fluid thereto.

13. The fluid analysis system according to claim 1, further comprising:

a channel fluidly coupled with at least one of said plurality of through-hole ports.

14. The fluid analysis system according to claim 1 wherein each of said plurality of through-hole ports defines a diameter, at least along a portion thereof, between about 0.1 mm and 5 mm.

15. The fluid analysis system according to claim 1 wherein each of said plurality of through-hole ports defines a diameter, at least along a portion thereof, between about 0.5 mm and 2 mm.

16. A fluid analysis system comprising:

a substrate having a first surface and a second surface; and

a plurality of through-hole ports extending through said substrate, each of said plurality of through-hole ports having a first open end at said first surface and a second open end at said second surface, each of said plurality of through-hole ports defining a varying cross-sectional dimension, each of said plurality of through-hole ports being sized to retain said test fluid therein at least in part by capillary force.

17. The fluid analysis system according to claim 16 wherein each of said plurality of through-hole ports defines a tapered cross-section from said first open end to said second open end.

18. The fluid analysis system according to claim 16 wherein each of said plurality of through-hole ports defines a first cross-section adjacent said first open end, a second cross-section adjacent said second open end, and a third cross-section at a position between said first open end and said second open end, said third cross-section being greater than at least one of said first cross-section and said second cross-section.

19. The fluid analysis system according to claim 16 wherein each of said plurality of through-hole ports defines a first cross-section adjacent said first open end, a second cross-section adjacent said second open end, and a third cross-section at a position between said first open end and said second open end, said third cross-section being less than at least one of said first cross-section and said second cross-section.

20. The fluid analysis system according to claim 16 wherein each of said plurality of through-hole ports defines a circular cross-section having a varying diameter.

21. The fluid analysis system according to claim 16 wherein each of said plurality of through-hole ports defines a rectangular cross-section having a varying cross-sectional dimension.

22. The fluid analysis system according to claim 16 wherein each of said plurality of through-hole ports defines a total volume between said first surface and said second surface of less than 20 μ

L.

23. The fluid analysis system according to claim 16 wherein each of said plurality of through-hole ports defines a total volume between said first surface and said second surface between about 0.5 μ

L and 10 μ

L.

24. The fluid analysis system according to claim 16 wherein a first of said plurality of through-hole ports receives a first test fluid and a second of said plurality of through-hole ports receives a second test fluid, said second test fluid being different than said first test fluid.

25. The fluid analysis system according to claim 16 wherein said capillary force is greater than the force of gravity.

26. The fluid analysis system according to claim 16 wherein each of said plurality of through-hole ports defines a diameter, at least along a portion thereof, between about 0.1 mm and 5 mm.

27. The fluid analysis system according to claim 16 wherein each of said plurality of through-hole ports defines a diameter, at least along a portion thereof, between about 0.5 mm and 2 mm.

28. A fluid analysis system comprising:

a substrate having a first surface and a second surface;

a plurality of through-hole ports extending through said substrate, each of said plurality of through-hole ports having a first open end at said first surface and a second open end at said second surface, each of said plurality of through-hole ports defining a varying cross-sectional dimension, each of said plurality of through-hole ports being sized to retain said test fluid therein at least in part by capillary force; and

a first hydrophobic coating disposed on said first surface, said first hydrophobic coating inhibiting cross-contamination of said test fluid in said plurality of through-hole ports along said first surface.

29. The fluid analysis system according to claim 28, further comprising:

a second coating applied within at least some of the plurality of through-hole ports, said second coating being different from said first hydrophobic coating.

30. The fluid analysis system according to claim 28 wherein each of said plurality of through-hole ports defines a tapered cross-section from said first open end to said second open end.

31. The fluid analysis system according to claim 28 wherein each of said plurality of through-hole ports defines a first cross-section adjacent said first open end, a second cross-section adjacent said second open end, and a third cross-section at a position between said first open end and said second open end, said third cross-section being greater than at least one of said first cross-section and said second cross-section.

32. The fluid analysis system according to claim 28 wherein each of said plurality of through-hole ports defines a first cross-section adjacent said first open end, a second cross-section adjacent said second open end, and a third cross-section at a position between said first open end and said second open end, said third cross-section being less than at least one of said first cross-section and said second cross-section.

33. The fluid analysis system according to claim 28 wherein each of said plurality of through-hole ports defines a circular cross-section having a varying diameter.

34. The fluid analysis system according to claim 28 wherein each of said plurality of through-hole ports defines a rectangular cross-section having a varying cross-sectional dimension.

35. The fluid analysis system according to claim 28 wherein each of said plurality of through-hole ports defines a total volume between said first surface and said second surface of less than 20 μ

L.

36. The fluid analysis system according to claim 28 wherein each of said plurality of through-hole ports defines a total volume between said first surface and said second surface between about 0.5 μ

L and 10 μ

L.

37. The fluid analysis system according to claim 28 wherein a first of said plurality of through-hole ports receives a first test fluid and a second of said plurality of through-hole ports receives a second test fluid, said second test fluid being different than said first test fluid.

38. The fluid analysis system according to claim 28 wherein said capillary force is greater than the force of gravity.

39. The fluid analysis system according to claim 28 wherein each of said plurality of through-hole ports defines a diameter, at least along a portion thereof, between about 0.1 mm and 5 mm.

40. The fluid analysis system according to claim 28 wherein each of said plurality of through-hole ports defines a diameter, at least along a portion thereof, between about 0.5 mm and 2 mm.