Compartmentalised screening by microfluidic control
First Claim
1. A method for identifying a compound or compounds in a repertoire of compounds, which compound or compound(s) possess(es) a desired activity, comprising the steps of:
- a) compartmentalising the compounds into microcapsules, such that only a subset of the repertoire is represented in multiple copies in any one microcapsule; and
b) identifying the compounds which possess the desired activity, wherein one or both of steps a) and b) is performed under microfluidic control.
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Abstract
The invention describes a method for the identification of compounds which bind to a target component of a biochemical system or modulate the activity of the target, comprising the steps of: a) compartmentalising the compounds into microcapsules together with the target, such that only a subset of the repertoire is represented in multiple copies in any one microcapsule; and b) identifying the compound which binds to or modulates the activity of the target; wherein at least one step is performed under microfluidic control. The invention enables the screening of large repertoires of molecules which can serve as leads for drug development.
337 Citations
5 Claims
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1. A method for identifying a compound or compounds in a repertoire of compounds, which compound or compound(s) possess(es) a desired activity, comprising the steps of:
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a) compartmentalising the compounds into microcapsules, such that only a subset of the repertoire is represented in multiple copies in any one microcapsule; and
b) identifying the compounds which possess the desired activity, wherein one or both of steps a) and b) is performed under microfluidic control.
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2. A method for preparing a compound, comprising the steps of:
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(a) compartmentalising the compounds into microcapsules, such that only a subset of the repertoire is represented in multiple copies in any one microcapsule;
(b) identifying the compounds which possess the desired activity. (c) identifying and producing the compound having the desired activity, wherein one or more of steps a, b and c is performed under microfluidic control.
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3. A method for preparing a compound, comprising the steps of:
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(a) compartmentalising the compounds into microcapsules, such that only a subset of the repertoire is represented in multiple copies in any one microcapsule;
(b) identifying and, optionally, sorting the microcapsules which have contain the compound(s) having the desired activity using a change in their optical properties; and
(c) identifying and producing the compound having the desired activity.
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4. A method for screening a compound or compounds capable of modulating the activity of a target, comprising the steps of:
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(a) compartmentalising the compounds into microcapsules, such that only a subset of the repertoire is represented in multiple copies in any one microcapsule;
(b) identifying and, optionally, sorting the microcapsules which contain the compound(s) having the desired activity using a change in their optical properties; and
(c) contacting a target having a desired activity with the compound or compounds and monitoring the modulation of an activity of the target by the compound or compounds, wherein one or more of steps a, b and c is performed under microfluidic control.
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5. A method for preparing a target comprising the steps of:
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(a) providing a synthesis protocol for a target wherein at least one step is facilitated by a compound;
(b) preparing variants of the compound which facilitates this step;
(c) compartmentalising the compounds into microcapsules, such that only a subset of the repertoire is represented in multiple copies in any one microcapsule;
(d) identifying and, optionally, sorting the microcapsules which contain the compound(s) having the desired activity using a change in their optical properties; and
(e) preparing the target using the compound identified in (d) to facilitate the relevant step of the synthesis, wherein one or more of steps a to e is performed under microfluidic control.
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Specification