Process for treatment of protein samples

• US 20070092924A1
• Filed: 10/25/2005
• Published: 04/26/2007
• Est. Priority Date: 10/25/2005
• Status: Abandoned Application

• Current Assignee: Anderson Forschung Group, LLC
• Original Assignee: Anderson Forschung Group, LLC
• Inventors: Anderson, Norman
• Application Number:
  US11/256,946
• Publication Number:
  US 20070092924A1
• Time in Patent Office: Days
• US Class Current: 435/23
• CPC Class Codes: C12Q 1/37 involving peptidase or proteinase

Abstract

The process of the invention comprises 1) preparing a dry porous support incorporating one or more biomolecule-modifying reagents, 2) applying a biomolecule-containing sample solution to said support wherein the volume of said sample is equal to or less than the imbibition capacity of said support so that the sample is completely imbibed on said support, 3) allowing the product of step 2 sufficient time for said biomolecule-modifying reagent to interact with the biomolecule of interest in said sample, then 4) recovering biomolecules from said support and devices for use therewith

Claims

1. A process for treatment of biomolecules comprising the steps of:

1) preparing a porous support incorporating one or more biomolecule-modifying reagents,

2) applying a biomolecule-containing sample solution to said support wherein the volume of said sample is equal to or less than the imbibition capacity of said support so that the sample is completely imbibed into said support,

3) allowing the product of step 2 sufficient time for said biomolecule-modifying reagent to interact with the biomolecule of interest in said sample, then

4) recovering biomolecules from said support.

2. The process of claim 1 wherein, after step 3, the product of step 3 is stored before the recovery step.

3. The process of claim 2 wherein the product of step 3 is stored as a dried product before the recovery step.

4. The process of claim 1 wherein said sample is imbibed into said support by capillary action.

5. The process of claim 1 wherein said support contains at least one of particles, a membrane, a porous film, a woven or non-woven fabric, a sintered aggregate of particles, a foam or a monlithic support.

6. The process of claim 1 wherein said support comprises at least two phases.

11. The process of claim 1 wherein, in step 2, the biomolecule-containing sample is imbibed as said sample flows within the plane of said support.

12. The process of claim 1 wherein at least one of said reagents is formulated, coated or encapsulated to delay its action on said biomolecules relative to at least one other said reagent.

13. The process of claim 1 wherein said support incorporates at least one reagent chosen from denaturants, sulfhydral reducing agents, thiol-reactive compounds, proteolytic enzymes, protease inhibitors, detergents and salts.

14. The process of claim 1 wherein at least one said biomolecule-modifying reagent is bound to said support co-valently.

15. The process of claim 1 wherein at least one said biomolecule-modifying reagent is bound to said support non-covanlently

16. The process of claim 14 wherein at least one said biomolecule-modifying reagent is cross-linked after binding to said support

18. The process of claim 1 wherein, in step 1, at least one biomolecule-modifying reagent incorporated in said support is not bound to said support.

19. The process of claim 1 wherein, before step 2 a wetting agent is added to said sample before inbibition of step 3.

20. The process of claim 1 wherein at least one membrane is interposed between the sample and said support incorporating one or more biomolecule-modifying reagents.

7. A device for use in analysis of at least one biomolecule comprising a walled container with an exit opening spanned by a support, said support having therein at least one biomolecule modifying reagent, said support having imbibed therein a cell-free biomolecule-containing sample wherein the volume of the total sample applied to said support in said walled container is less than or equal to the imbibition capacity of said support.

10. The device of claim 7 wherein the support comprises at least one of the biomolecule-modifying reagents on support materials chosen from particles, membranes, porous films, woven or non-woven fabric, sintered aggregate of particles, foam or monolithic support.

17. The device of claim 7 wherein one biomolecule-modifying reagent is an enzyme wherein said enzyme is bound to said support.

8. A device for use in analysis of a biomolecule-containing material comprising a filter impermiable to cells in contact with a support within which there is incorporated in said support at least one biomolecule-modifying agents.

9. A device of claim 8 wherein ay least one biomolecule-modifying agent is a digesting or denaturing agent.