Fermentative production of lipids on an industrial scale using chemically defined media

• US 20070092955A1
• Filed: 12/14/2006
• Published: 04/26/2007
• Est. Priority Date: 02/20/1997
• Status: Abandoned Application

• Current Assignee: DSM IP Assets BV (DSM-Firmenich AG)
• Original Assignee: DSM IP Assets BV (DSM-Firmenich AG)
• Inventors: De Laat, Wilhelmus Theodorus Antonius, Koekman, Bertus, Preusting, Johannes Cornelis
• Application Number:
  US11/638,564
• Publication Number:
  US 20070092955A1
• Time in Patent Office: Days
• US Class Current: 435/134
• CPC Class Codes: C12N 9/16 acting on ester bonds (3.1)
• CPC Class Codes: C12N 9/2408 acting on alpha -1,4-glucosidic bonds
• CPC Class Codes: C12N 9/92 Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
• CPC Class Codes: C12P 17/06 containing a six-membered hetero ring, e.g. fluorescein
• CPC Class Codes: C12P 17/188 Heterocyclic compound containing in the condensed system at least one hetero ring having nitrogen atoms and oxygen atoms as the only ring heteroatoms ergot-alcaloids C12P17/183
• CPC Class Codes: C12P 19/62 the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin
• CPC Class Codes: C12P 23/00 Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes containing heterorings C12P17/00
• CPC Class Codes: C12P 35/00 Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
• CPC Class Codes: C12P 37/00 Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin
• CPC Class Codes: C12P 7/6472 containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence

Abstract

We describe the use of chemically defined media for the fermentative production of valuable compounds on an industrial scale. Microbial strains which are suitable for fermentation on an industrial scale using a chemically defined medium include fungal, yeast and bacterial strains. Suitable strains can be obtained as wild type strains or by screening and selection after mutagenic treatment or DNA transformation.

Claims

1. A process for production of a valuable compound, comprising:

(a) fermenting a microbial strain in a chemically defined medium and

(b) recovering of the valuable compound from the medium.

2. The process of claim 1, wherein the chemically defined medium comprises a carbon source and/or a nitrogen source.

3. The process of claim 1, wherein the chemically defined medium comprises a carbon source selected from the group consisting of carbohydrates such as glucose, lactose, fructose, sucrose, maltodextrins, starch and inulin, glycerol, vegetable oils, hydrocarbons, alcohols such as methanol and ethanol, organic acids such as acetate and higher alkanoic acids, and a nitrogen source selected from the group consisting of urea, ammonia, nitrate, ammonium salts such as ammonium sulphate, ammonium phosphate and ammonium nitrate, and amino acids such as glutamate and lysine.

4. The process of claim 1, wherein the chemically defined medium comprises (i) glucose as a carbon source and (ii) ammonia, an ammonium salt, or both as a nitrogen source.

5. The process of claim 1, wherein fermentation occurs via a batch, a repeated batch, a fed-batch, a repeated fed-batch, or a continuous fermentation process.

6. The process of claim 5, wherein fermentation occurs via a fed-batch process.

7. The process of claim 6, wherein a carbon source, a nitrogen source, or both are fed to the process.

8. The process of claim 7, wherein the carbon source is glucose and the nitrogen source is ammonia and/or an ammonium salt.

9. The process of claim 1, wherein the microbial strain is a filamentous strain.

10. The process of claim 9, wherein the filamentous strain is a fungus.

11. The process of claim 10, wherein the fungus is a Mucorales strain.

12. The process of claim 11, wherein the Mucorales strain is a Mortierella strain.

13. The process of claim 12, wherein the Mucorales strain is Mortierella alpina and the valuable compound is a lipid comprising arachidonic acid.

14. The process of claim 13, wherein the lipid comprising arachidonic acid is a triglyceride.

15. A method for preparing and/or improving a microbial strain producing a valuable compound of interest which is capable of being fermented in a chemically defined medium, comprising:

(a) subjecting a parent strain to a mutagenic treatment and/or to DNA transformation to obtain mutants and/or transformants;

(b) screening the mutants and/or transformants for their growth performance on a chemically defined medium and their production level of said valuable compound of interest; and

(c) selecting mutants and/or transformants which have a good growth performance on the chemically defined medium and/or an improved production level of said valuable compound of interest as compared to the parent strain.

16. The method of claim 15, wherein the parent strain is selected from the group consisting of strains which have a good growth performance on a chemically defined medium, but which need to be improved on production level.

17. The method of claim 15, wherein the parent strain is selected from the group consisting of strains which have a high production level of a desired compound but a relatively bad growth performance on a chemically defined medium.

18. Use of a chemically defined fermentation medium for the production of a valuable compound by fermentation of a microbial strain on an industrial scale.