FORENSIC TEST FOR HUMAN SALIVA
First Claim
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1. A device for detecting human saliva, comprising:
- a) a wettable material, b) a sample comprising a first human salivary amylase (hAMY1A) antibody, where said first antibody is labeled for detection, and c) a second hAMYlA antibody immobilized to said wettable material at a single location, wherein the first antibody and the second antibody can simultaneously bind to human salivary amylase, but do not bind pancreatic amylase or non-human salivary amylase.
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Abstract
Lateral flow immunochromatographic strip tests for the detection of human saliva, method of detecting human saliva, and methods of manufacturing ICS tests for the detection of human saliva are described.
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Citations
23 Claims
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1. A device for detecting human saliva, comprising:
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a) a wettable material, b) a sample comprising a first human salivary amylase (hAMY1A) antibody, where said first antibody is labeled for detection, and c) a second hAMYlA antibody immobilized to said wettable material at a single location, wherein the first antibody and the second antibody can simultaneously bind to human salivary amylase, but do not bind pancreatic amylase or non-human salivary amylase. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method for detecting the presence of human saliva comprising:
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a) swabbing a surface suspected of containing saliva with a swab;
b) resuspending the contents of the swab (a) in buffer;
c) applying buffer (b) to a device for detecting human saliva comprising;
i) a first human salivary amylase (hAMY1A) antibody labeled for detection, and ii) a second hAMY1A antibody immobilized to a wettable material at a single separate location, wherein the first antibody and the second antibody can simultaneously bind to human salivary amylase, but do not bind pancreatic amylase or non-human salivary amylase; and
d) detecting the presence or absence of concentrated detection antibodies at the second immobilized antibody wherein the presence of a test line confirms the presence of human saliva. - View Dependent Claims (11, 12, 13, 14, 15, 16)
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17. A method of manufacturing one or more human salivary immunochromatographic strip (ICS) tests comprising:
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a) placing a backing on one surface;
b) adhering to the backing;
i) a first pad comprising a first human salivary amylase (hAMY1A) antibody labeled for detection, ii) a second pad opposite the first pad, and iii) a membrane comprising a second hAMY1A antibody immobilized in a test line wherein said chromatographic membrane contacts both first and second pad, wherein the first antibody and the second antibody can simultaneously bind to human salivary amylase, but do not bind pancreatic amylase or non-human salivary amylase;
c) laminating said components to said backing creating an assembled card; and
d) cutting said assembled card into longitudinal strips to create one or more ICS test strips. - View Dependent Claims (18, 19, 20, 21, 22, 23)
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Specification