Evanescent wave sensor with attached ligand
First Claim
1. A method of making an evanescent wave sensor, the method comprising contacting a first reactive moiety having the structure with a second reactive moiety having the structure
R2-X1Hm under conditions sufficient to result in a compound having the structure wherein:
- one of R1 or R2 is a sensor substrate;
the other of R1 or R2 is selected from a ligand, a linking group bound to a ligand, a functional group for binding a ligand, or a linking group bound to a functional group for binding a ligand;
X1 is selected from N or S; and
m equals 1 when X1 is N, or m equals 0 when X1 is S.
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Abstract
The invention in particular embodiments provides an evanescent wave sensor which includes a ligand bound to a sensor substrate via a β-hydroxy-linker moiety, as defined herein. Methods of making the subject evanescent wave sensors are also provided which include contacting a first reactive moiety which has an epoxy-ring moiety with a second reactive moiety which has an amino moiety or a thiol moiety, as further described herein. Also provided by the invention are methods in which a subject evanescent wave sensor is contacted with a sample, and binding of analytes in the sample to the sensor is assessed by evanescent wave detection. The invention also provides kits and systems for performing the subject methods.
20 Citations
24 Claims
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1. A method of making an evanescent wave sensor, the method comprising
contacting a first reactive moiety having the structure with a second reactive moiety having the structure
R2-X1Hmunder conditions sufficient to result in a compound having the structure wherein: -
one of R1 or R2 is a sensor substrate;
the other of R1 or R2 is selected from a ligand, a linking group bound to a ligand, a functional group for binding a ligand, or a linking group bound to a functional group for binding a ligand;
X1 is selected from N or S; and
m equals 1 when X1 is N, or m equals 0 when X1 is S. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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- 13. An evanescent wave sensor having the structure:
Specification