Nucleic acid array

• US 20070099187A1
• Filed: 05/13/2004
• Published: 05/03/2007
• Est. Priority Date: 05/14/2003
• Status: Abandoned Application

• Current Assignee: Canon Ayutthaya Limited (Canon Inc.)
• Original Assignee: Canon Ayutthaya Limited (Canon Inc.)
• Inventors: Tsukada, Mamoru
• Application Number:
  US10/556,508
• Publication Number:
  US 20070099187A1
• Time in Patent Office: Days
• US Class Current: 435/6.11
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 2525/117 incorporating modified base
• CPC Class Codes: C12Q 2525/119 incorporating abasic sites
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers

Abstract

A nucleic acid array is provided that can efficiently perform analysis, and further, can estimate the number of expressions of amino acid mutations for all cases by use of a smaller number of arrays. The nucleic acid array has a plurality of oligonucleotides immobilized on a substrate, wherein the oligonucleotide has a sequence site that forms base pairs with at least two bases of the four bases (A, T, C, G or U) or has a sequence site that does not form a base pair with any of the four bases.

Claims

1. A nucleic acid array having a plurality of oligonucleotides immobilized on a substrate, wherein the oligonucleotide has a sequence site that forms base pairs with at least two bases of the four bases (A, T, C, G or U) or has a sequence site that does not form a base pair with any of the four bases.

2. The nucleic acid array according to claim 1, wherein the sequence site is a sequence site of a unit comprising a backbone having no base or a sequence site having a base selected from the group consisting of inosine, P-amino, P-imino, K-amino, K-imino, 5-Nitroindole, and 3-Nitropyrrole.

3. The nucleic acid array according to claim 1, wherein the sequence site is a sequence site of a unit comprising a backbone having no base.

4. The oligonucleotide array according to claim 1, wherein in a partial sequence of the oligonucleotide, successive triplets correspond to codons of amino acids and a third base of the codon forms the sequence site.

5. A detection method comprising detecting a target nucleic acid using the nucleic acid array set forth in claim 1.

6. A method of distinguishing an amino acid mutated sequence, comprising distinguishing between a nucleic acid mutated sequence not accompanied by an amino acid mutation and a nucleic acid mutated sequence accompanied by an amino acid mutation through hybridization, using the nucleic acid array set forth in claim 1.