Probe/target stabilization with add-in oligo
First Claim
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1. A method of analyzing a sample comprising small RNAs, the method comprising:
- contacting an array with the sample in the presence of an add-in oligo, the array comprising a set of probes and an array support, each probe comprising an add-in oligo complementary region bound to the array support and a target complementary region directly bound to the add-in oligo complementary region, the target complementary region bound to the array support via the add-in oligo complementary region; and
interrogating the array to obtain information about small RNAs in the sample.
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Abstract
The invention relates to analyzing a sample containing small RNAs. In exemplary embodiments, the sample is contacted with an array in the presence of an add-in oligo. In typical embodiments, probes on the array include regions that are complementary to small RNAs and regions that are complementary to the add-in oligo. The array is then interrogated to obtain information about small RNA in the sample. Arrays and kits in accordance with the invention are also described.
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Citations
22 Claims
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1. A method of analyzing a sample comprising small RNAs, the method comprising:
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contacting an array with the sample in the presence of an add-in oligo, the array comprising a set of probes and an array support, each probe comprising an add-in oligo complementary region bound to the array support and a target complementary region directly bound to the add-in oligo complementary region, the target complementary region bound to the array support via the add-in oligo complementary region; and
interrogating the array to obtain information about small RNAs in the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 22)
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- 16. An array comprising a set of probes and an array support, each probe of the set of probes comprising an add-in oligo complementary region bound to the array support and a target complementary region directly bound to the add-in oligo complementary region, the target complementary region bound to the array support via the add-in oligo complementary region.
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21. A kit comprising:
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an array comprising a set of probes and an array support, each probe of the set of probes comprising an add-in oligo complementary region bound to the array support and a target complementary region directly bound to the add-in oligo complementary region, the target complementary region bound to the array support via the add-in oligo complementary region; and
an add-in oligo.
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Specification