Quality control of probe attachment on electrodes
First Claim
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1. A method of quantifying nucleic acid probes comprising:
- providing an electrode surface;
immobilizing a plurality of nucleic acid probes on said electrode surface;
providing a redox moiety capable of electrostatic association with said plurality of nucleic acid probes; and
detecting a total electrical current transferred by said redox moiety, wherein said total electrical current comprises a diffusion current and an adsorption current, wherein said detection occurs at a frequency at which said adsorption current is greater than said diffusion current.
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Abstract
The present disclosure relates to methods of quantifying nucleic acid molecules immobilized on an electrode of an electrochemical assay chip. Such quantification can be used in testing and quality control of assay chips. Materials and instrumentation useful in performing such techniques are also disclosed.
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13 Claims
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1. A method of quantifying nucleic acid probes comprising:
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providing an electrode surface;
immobilizing a plurality of nucleic acid probes on said electrode surface;
providing a redox moiety capable of electrostatic association with said plurality of nucleic acid probes; and
detecting a total electrical current transferred by said redox moiety, wherein said total electrical current comprises a diffusion current and an adsorption current, wherein said detection occurs at a frequency at which said adsorption current is greater than said diffusion current. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method of evaluating the quality of a nucleic acid assay device comprising:
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providing a nucleic acid assay device comprising an electrode surface wherein a plurality of nucleic acid probes are immobilized on said electrode surface;
quantitating said nucleic acid probes by detecting an electrical signal;
hybridizing a plurality of nucleic acid targets to said nucleic acid probes;
quantitating said nucleic acid targets by detecting an electrical signal;
correlating the quantity of said nucleic acid probes with the quantity of said nucleic acid targets to evaluate the quality of the nucleic acid assay device.
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12. A method for performing an electrochemical assay, comprising:
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providing an assay device comprising at least one electrode having polynucleotide capture probe attached;
providing a redox moiety in solution in contact with the electrode, wherein the redox moiety is capable of association with both the capture probe and with a polynucleotide target capable of hybridizing with the capture probe;
quantitating the capture probe on the electrode by measuring current associated with redox interaction with the electrode using cyclic voltammetry at a frequency greater than 75 Hz;
thereafter contacting the capture probe with a liquid to be analyzed for the presence of the polynucleotide target;
measuring a target current associated with interaction between the electrode and redox moiety associated with the polynucleotide target; and
calibrating the result of the target current measurement with reference to the quantity of polynucleotide capture probe on the electrode.
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13. A method for performing an electrochemical assay, comprising the steps of:
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providing an assay device having a capture probe attached to an electrode;
providing device-specific data reflective of the quantity of capture probe on the electrode, as measured electrochemically;
performing an electrochemical assay to detect an analyte, thereby generating a value reflective of the quantity of the analyte; and
providing a result of the assay reflecting interpretation of the result in light of the device-specific data.
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Specification