High throughput mobility shift
First Claim
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1. A method of detecting an interaction between a first molecule and at least a second molecule in a microfluidic device, the method comprising:
- a) flowing a first labeled molecule with a first electrophoretic mobility, through at least a first microchannel;
b) flowing at least a second molecule with a second electrophoretic mobility through the microchannel;
c) applying a pulsed electric field through the microchannel, wherein the pulsed electric field separates the first and at least second molecule based upon the electrophoretic mobilities of the molecules; and
, d) detecting a level of label or a level of signal from a label in the microchannel over a select time period.
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Abstract
The present invention provides novel microfluidic devices and methods for performing pulsed field mobility shift assays in microfluidic devices. In particular the devices and methods of the invention utilize differences between electrophoretic mobilities (e.g., as between reactants and products, especially in non-fluorogenic reactions) in order to separate the species and thus analyze the reaction.
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Citations
42 Claims
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1. A method of detecting an interaction between a first molecule and at least a second molecule in a microfluidic device, the method comprising:
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a) flowing a first labeled molecule with a first electrophoretic mobility, through at least a first microchannel;
b) flowing at least a second molecule with a second electrophoretic mobility through the microchannel;
c) applying a pulsed electric field through the microchannel, wherein the pulsed electric field separates the first and at least second molecule based upon the electrophoretic mobilities of the molecules; and
,d) detecting a level of label or a level of signal from a label in the microchannel over a select time period. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42)
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Specification