Compositions for detection of a target nucleic acid sequence
First Claim
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1. A composition comprising a reverse transcriptase, a cleavage means, a first oligonucleotide and a second oligonucleotide.
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Abstract
The invention relates to compositions for generating a signal indicative of the presence of a target nucleic acid in a sample. The compositions include a reverse transcriptase, a nuclease, an upstream primer and downstream probe.
47 Citations
30 Claims
- 1. A composition comprising a reverse transcriptase, a cleavage means, a first oligonucleotide and a second oligonucleotide.
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23. A composition comprising:
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(i) a target nucleic acid, which comprises in 3′
to 5′
order a first region, an extension region, and a second region;
(ii) a first oligonucleotide that is at least partially complementary to said first region of said target nucleic acid;
(iii) a second oligonucleotide comprising a 5′
region and a 3′
region, wherein said 3′
region is at least partially complementary to said second region of said target nucleic acid and wherein said 5′
region is not complementary to said target nucleic acid;
(iv) a reverse transcriptase; and
(v) a cleavage means. - View Dependent Claims (24)
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25. A composition comprising:
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(i) a target nucleic acid, which comprises in 3′
to 5′
order a first region, an extension region, and a second region;
(ii) a first oligonucleotide that is at least partially complementary to said first region of said target nucleic acid;
(iii) a second oligonucleotides corresponding to said target nucleic acids, said second oligonucleotides comprising a 5′
region and a 3′
region, wherein said 3′
region is at least partially complementary to said second region of said target nucleic acid and wherein said 5′
region is at least partially complementary to a region that may be upstream of, downstream of, or comprise said first region of said target nucleic acid;
(iv) a reverse transcriptase; and
(v) a cleavage means. - View Dependent Claims (26)
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27. A composition comprising:
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(i) a template nucleic acid, which comprises in 3′
to 5′
order a first region, an extension region, and a second region;
(ii) a first oligonucleotide that is at least partially complementary to said first region of said target nucleic acid;
(iii) a second oligonucleotide comprising a 5′
region and a 3′
region, wherein said 3′
region is at least partially complementary to said second region of said target nucleic acid and wherein said 5′
region is not complementary to said target nucleic acid;
(iv) a reverse transcriptase; and
(v) a cleavage means. - View Dependent Claims (28)
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29. A composition comprising:
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(i) a template nucleic acid, which comprises in 3′
to 5′
order a first region, an extension region, and a second region;
(ii) a first oligonucleotide that is at least partially complementary to said first region of said template nucleic acid;
(iii) a second oligonucleotides corresponding to said template nucleic acids, said second oligonucleotides comprising a 5′
region and a 3′
region, wherein said 3′
region is at least partially complementary to said second region of said template nucleic acid and wherein said 5′
region is at least partially complementary to a region that may be upstream of, downstream of, or comprise said first region of said template nucleic acid;
(iv) a reverse transcriptase; and
(v) a cleavage means. - View Dependent Claims (30)
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Specification