Pixel arrays

• US 20070099240A1
• Filed: 11/10/2006
• Published: 05/03/2007
• Est. Priority Date: 02/16/2001
• Status: Abandoned Application

• Current Assignee: Pepscan Systems B.V. (Biosynth Carbosynth)
• Original Assignee: Pepscan Systems B.V. (Biosynth Carbosynth)
• Inventors: van Dijk, Evert, Slootstra, Jelle, Puijk, Wouter
• Application Number:
  US11/595,775
• Publication Number:
  US 20070099240A1
• Time in Patent Office: Days
• US Class Current: 435/7.1
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00707 separated from the reactor apparatus
• CPC Class Codes: G01N 33/544 the carrier being organic
• CPC Class Codes: G01N 33/545 Synthetic resin

Abstract

The invention relates to the detection of biomolecules or analogs of the biomolecules in micro-arrays and the supports used for the micro-arrays. Methods for determining or testing binding of a first member molecule within an array or library of tentative first member binding molecules for binding with a second member binding molecule are disclosed. A support for a micro-array suitable for determining binding of a first member molecule within a library of spots of tentative first member binding molecules with a second member binding molecule is disclosed. The support includes a support surface wherein surface patches are interspersed within surface areas that are materially distinct from the patches.

Claims

1-6. -6. (canceled)

7. A process for determining binding of a first member molecule within a library of tentative first member binding molecules with a second member binding molecule, the process comprising:

providing a micro-array support with positioned droplets or spots comprising the tentative first member binding molecules, the micro-array support comprising;

a support surface having surface areas; and

surface patches interspersed within the surface areas;

wherein the surface areas are materially distinct from the surface patches and wherein the patches are smaller in at least one or two dimensions than the size of the circumference of the positioned droplets or spots;

providing a second member binding molecule; and

detecting binding of the first member molecule with the second member binding molecule.

8. The process according to claim 7, wherein the binding is detected with an optically detectable marker.

9. The process according to claim 8, wherein the optically detectable marker comprises a fluorophore.

10. The process according to claim 9, wherein the binding is detected with an enzyme-linked-assay.

11. The process according to claim 10, wherein the enzyme-linked-assay comprises the production of a fluorescent substrate.

12. The process according to claim 11, wherein an enzyme of the enzyme-linked-assay comprises alkaline phosphatase.

13. The process according to claim 7, wherein the library of spots includes a density of at least twenty-five spots per square centimeter on the support surface of the micro-array support.

14-23. -23. (canceled)

24. A process for identifying or obtaining a synthetic molecule having a binding site, the process comprising:

providing a micro-array support with positioned droplets or spots comprising the tentative first member binding molecules, the micro-array support comprising;

a support surface having surface areas; and

surface patches interspersed within the surface areas;

wherein the surface areas are materially distinct from the surface patches and wherein the patches are smaller in at least one or two dimensions than the size of the circumference of the positioned droplets or spots;

providing a second member binding molecule; and

detecting binding of the first member molecule with the second member binding molecule.

25. A process for identifying or obtaining a binding molecule capable of binding to a binding site, the process comprising:

providing a micro-array support with positioned droplets or spots comprising the tentative first member binding molecules, the micro-array support comprising;

a support surface having surface areas; and

surface patches interspersed within the surface areas;

wherein the surface areas are materially distinct from the surface patches and wherein the patches are smaller in at least one or two dimensions than the size of the circumference of the positioned droplets or spots;

providing a second member binding molecule; and

detecting binding of the first member molecule with the second member binding molecule.

26. (canceled)