Compositions and liquid crystals

• US 20070099249A1
• Filed: 08/25/2006
• Published: 05/03/2007
• Est. Priority Date: 08/25/2005
• Status: Active Grant

• Current Assignee: Platypus Technologies, LLC
• Original Assignee: Platypus Technologies, LLC
• Inventors: Murphy, Christopher, Abbott, Nicholas, Israel, Barbara
• Granted Patent: US 7,662,572 B2
• Time in Patent Office: Days
• US Class Current: 435/7.5
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C07D 311/24 with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached in position 2
• CPC Class Codes: C07D 495/04 Ortho-condensed systems
• CPC Class Codes: C07K 14/001 by chemical synthesis
• CPC Class Codes: C07K 16/1063 env, e.g. gp41, gp110/120, gp160, V3, PND, CD4 binding site
• CPC Class Codes: C07K 2317/56 variable (Fv) region, i.e. VH and/or VL
• CPC Class Codes: C09K 19/3402 having oxygen as hetero atom sugars C09K19/0422
• CPC Class Codes: C09K 2019/3425 Six-membered ring with oxygen(s) in fused, bridged or spiro ring systems
• CPC Class Codes: G01N 33/54353 with ligand attached to the carrier via a chemical coupling agent coatings G01N33/54393
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/551 the carrier being inorganic

Abstract

The present invention relates to compounds, compositions, and methods in the field of detection of analytes. In particular to detection of viruses, cells, bacteria, lipid-membrane containing organisms, proteins, nucleic acids, carbohydrates and other biomolecules, organic molecules and inorganic molecules using a liquid crystal assay format.

Claims

1. A compound comprising a recognition moiety substituted with a mesogen substituent.

2. The compound of claim 1, wherein said mesogen is an organic molecule.

3. The compound of claim 1, wherein said mesogen is selected from the group consisting of 5,5′

-[(2-hydroxytrimethylene)dioxy]bis(4-oxo-4H-1-benzopyran-2-carboxylic acid), 4′

-alkyl-(1,1′

-Biphenyl)-4-carbonitrile, and 5,5′

-[(2-hydroxytrimethylene)dioxy]bis(4-oxo-4H-1-benzopyran-2-carboxylic acid).

4. A compound of claim 1, wherein the mesogen is an amino acid sequence.

5. A compound of claim 1, wherein the recognition moiety is an amino acid sequence.

6. A device comprising the compound of claim 1.

7. The device of claim 6, wherein said device comprises a substrate having a surface in contact with said compound.

8. The device of claim 6, wherein said surface is anisotropic.

9. A liquid crystal composition comprising a plurality of compounds comprising a recognition moiety substituted with a mesogen substituent.

10. The composition of claim 9, wherein the recognition moiety is biotin.

11. The composition of claim 9, wherein the mesogen is selected from the group consisting of 5,5′

-[(2-hydroxytrimethylene)dioxy]bis(4-oxo-4H-1-benzopyran-2-carboxylic acid), 4′

-alkyl-(1,1′

-Biphenyl)-4-carbonitrile and 5,5′

-[(2-hydroxytrimethylene)dioxy]bis(4-oxo-4H-1-benzopyran-2-carboxylic acid).

12. The composition of claim 9, further comprising a metal salt.

13. A liquid crystal composition of claim 12, wherein said metal is selected from the group consisting of copper, indium, and chromium.

14. A device comprising the composition of claim 9.

15. The device of claim 14, wherein said device comprises a substrate having a surface in contact with said compound.

16. The device of claim 15, wherein said surface is anisotropic.

17. A method comprising:

a) providing the device of claim 15 and a sample suspected of containing an analyte that is capable of interacting with said recognition moiety;

b) contacting said device with said sample, wherein a change in the orientation of said liquid crystal composition is indicative of the presence of said analyte.

18. A detection method comprising:

a) providing;

i) an analyte;

ii) an device comprising an optically transparent substrate comprising a detection region comprising a recognition moiety, said detection region comprising a series of lines readable by an optical scanner; and

iii) a liquid crystal composition

b) in any order, contacting said analyte with said device and said device with said liquid crystal composition;

c) reading said series of lines readable by an optical scanner.