Method for detecting analytes by means of an analyte/polymeric activator bilayer arrangement

• US 20070105119A1
• Filed: 10/25/2004
• Published: 05/10/2007
• Est. Priority Date: 10/29/2003
• Status: Abandoned Application

• Current Assignee: Agency For Science Technology and Research (Government of Singapore)
• Original Assignee: Agency For Science Technology and Research (Government of Singapore)
• Inventors: Zhang, Chunyan, Yu, Yuan, Hong, Xie, Gao, Zhinqiang
• Application Number:
  US10/577,293
• Publication Number:
  US 20070105119A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: C12Q 1/26 involving oxidoreductase
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: C12Q 2563/113 the label being electroactive, e.g. redox labels
• CPC Class Codes: C12Q 2565/519 characterised by the capture moiety being a single stranded oligonucleotide
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding

Abstract

The invention relates to the field of analytical sensors. In particular, the invention relates to a method for the detection of analytes in a sample by means of an electrode arrangement, which is characterized by the formation of a conductive bilayer of analytes and an agent for increasing the conductivity of said analytes on the surface of an electrode. The invention is also directed to an electrode arrangement useful for performing such method as well as to the use of such electrode arrangement as biosensor. Also disclosed is a novel class of redox polymers that are suitable for being used in the electrochemical detection of analytes. A method of making this class of polymers is also disclosed.

Claims

15-1. The method of claim 15, wherein the capture molecule is at least on ligand capable of binding proteins or peptides.

17. A method for the electrochemical detection of an analyte molecule by means of a detection electrode, the method comprising:

(a) immobilizing capture molecules, which are capable of binding the analyte molecule to be detected, on the detection electrode;

(b) contacting the electrode with a solution supposed to contain the analyte molecule to be detected;

(c) allowing the analyte molecule contained in said solution to bind to the capture molecules on the electrode, thereby allowing formation of complexes between a capture molecule and an said complexes forming a first layer on the detection electrode;

(d) contacting the detection electrode with an electrochemical activator, wherein said electrochemical activator has an electrostatic net charge that is complementary to the electrostatic net charge of the complex formed between a capture molecule and an analyte molecule, thereby forming a second layer on the electrode, wherein the second layer and the first layer together form a conducting bilayer, and wherein the capture molecules are capable of transferring electrons to or from the electrochemical activator from or to the electrode, respectively;

(e) performing an electrical measurement at the detection electrode, and;

detecting the analytes by comparing the result of the electrical measurement obtained with that of a control measurement.

25. A biosensor for the electrochemical detection of an analyte molecule, comprising:

(a) an detection electrode;

(b) a first layer on the detection electrode comprising complexes between a capture molecule, which is capable of binding the analyte molecule to be detected, and an analyte molecule; and

(c) a second layer comprising an electrochemical activator, wherein said electrochemical activator has an electrostatic net charge that is complementary to the electrostatic net charge of the complex formed between a capture molecule and an analyte molecule, wherein the second layer and the first layer together form a conducting bilayer.

26-45. -45. (canceled)