Raised surface assay plate

• US 20070105185A1
• Filed: 05/13/2004
• Published: 05/10/2007
• Est. Priority Date: 05/16/2003
• Status: Active Grant

• Current Assignee: TransForm Pharmaceuticals, Inc. (Johnson & Johnson)
• Original Assignee: TransForm Pharmaceuticals, Inc. (Johnson & Johnson)
• Inventors: Lemmo, Anthony, Cima, Michael, Monagle, Julie, Pryce Lewis, Wendy, Gyory, J., Gonzalez-Zugasli, Javier
• Granted Patent: US 7,763,455 B2
• Time in Patent Office: Days
• US Class Current: 435/33
• CPC Class Codes: B01L 2300/0609 Holders integrated in container to position an object
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B01L 3/5088 confining liquids at a location by surface tension, e.g. virtual wells on plates, wires B01L3/50857 takes precedence

Abstract

The assay plate includes a substrate having an substrate surface and at least one raised pad extending from the substrate surface. The raised pad includes a substantially planar sample receiving surface configured for holding a sample thereon for in situ experimentation. The sample receiving surface preferably has at least one sharp edge at the junction between a sidewall coupling the sample receiving surface to the substrate surface. The sample receiving surface is preferably a circle, oval, square, rectangle, triangle, pentagon, hexagon, or octagon shape that is sized to hold a predetermined volume of the sample. A method of using the above described assay plate is also provided. Once a raised pad extending from a substrate is formed, a sample is deposited on the raised pad. Experiments are subsequently performed using the sample on the raised pad.

Claims

1-93. -93. (canceled)

94. A transdermal patch comprising:

a flexible substrate having a flexible surface;

at least two raised pads extending from said substrate surface and having a substantially planar sample receiving surface configured for holding a sample thereon for in vivo use.

95. The transdermal patch of claim 94, further comprising an array of samples supported by the planar sample receiving surface.

96. The transdermal patch of claim 95, wherein said array contains at least 8 samples.

97. The transdermal patch of claim 95, wherein said array contains at least 16 samples.

98. The transdermal patch of claim 95, wherein said array contains at least 32 samples.

99. The transdermal patch of claim 95, wherein said array contains at least 96 samples.

100. The transdermal patch of claim 95 wherein said array comprises samples spaced at least about 5 mm apart.

101. The transdermal patch of claim 96 wherein said array comprises samples spaced between about 5 mm and 50 mm apart.

102. The transdermal patch of claim 97 wherein said array comprises samples spaced between about 5 mm and 50 mm apart.

103. The transdermal patch of claim 98 wherein said array comprises samples spaced between about 5 mm and 20 mm apart.

104. The transdermal patch of claim 99 wherein said array comprises samples spaced between about 5 mm and 20 mm apart.

105. The transdermal patch of claim 94 wherein said raised pads are spaced between 5 and 15 mm apart.

106. An assay plate comprising:

a flexible substrate having a flexible surface;

at least two raised pads extending from said substrate surface and having a substantially planar sample receiving surface configured for holding a sample thereon for in vitro use.