NF-kappa B activating gene

• US 20070105198A1
• Filed: 01/08/2007
• Published: 05/10/2007
• Est. Priority Date: 12/28/2000
• Status: Active Grant

• Current Assignee: Akio Matsuda, Goichi Honda, Shuji Muramatsu, Yukiko Nagano
• Original Assignee: Akio Matsuda, Goichi Honda, Shuji Muramatsu, Yukiko Nagano
• Inventors: Matsuda, Akio, Nagano, Yukiko, Muramatsu, Shuji, Honda, Goichi
• Granted Patent: US 7,629,453 B2
• Time in Patent Office: Days
• US Class Current: 435/69.100
• CPC Class Codes: A61K 39/395 Antibodies agglutinins A61K38/36 ; as drug carriers A61K47/50; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
• CPC Class Codes: C07K 14/4702 Regulators; Modulating activity
• CPC Class Codes: C07K 14/4705 stimulating, promoting or activating activity
• CPC Class Codes: C07K 16/18 against material from animals or humans
• CPC Class Codes: C12N 2503/02 Drug screening
• CPC Class Codes: G01N 2500/00 Screening for compounds of potential therapeutic value
• CPC Class Codes: G01N 2800/24 Immunology or allergic disorders
• CPC Class Codes: G01N 33/5011 for testing antineoplastic activity
• CPC Class Codes: G01N 33/57484 involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
• CPC Class Codes: G01N 33/6872 Intracellular protein regulatory factors and their receptors, e.g. including ion channels
• CPC Class Codes: G01N 33/6893 related to diseases not provided for elsewhere
• CPC Class Codes: Y02A 90/10 Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation

Abstract

Provided are proteins having NF-κB activity, which are used for diagnosing, treating or preventing diseases associated with the excessive activation or inhibition of NF-κB. Using plasmid pNFκB-Luc, cDNA encoding a protein capable of activating NF-κB has been cloned from a cDNA library constructed from human lung fibroblasts, and the DNA sequence and the deduced amino acid sequence determined. The protein, the DNA encoding the protein, a recombinant vector containing the DNA, and a transforrnant containing the recombinant vector are useful for screening a substance inhibiting or promoting NF-κB activation.

Claims

1. An isolated polynucleotide which comprises a nucleotide sequence encoding a protein that activates NF-κ

B and which comprises an amino acid sequence represented by SEQ ID NO;

162 or an amino acid sequence 96% identical thereto over the entire length thereof.

5. The isolated polynucleotide of claim 1 that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:

162.

6. A recombinant vector which comprises a polynucleotide according to any one of claims 1 to 5.

7. A transformed cell which comprises the recombinant vector according to claim 6.

8. A process for producing a protein comprising,

(a) culturing a transformed cell comprising the isolated polynucleotide according to any one of claims 1 to 5 under conditions providing expression of the encoded protein;

and

(b) recovering the protein from the culture product.

9. A method for screening compounds in respect of activity to inhibit or promote NF-κ

B activation, which comprises the step of;

(a) providing a cell with a gene comprising a polynucleotide according to claim 1, 2, or 3 encoding a protein that activates NF-κ

B, and a component that provides a detectable signal associated with activation of NF-κ

B;

(b) culturing a transformed cell under conditions that permit the expression of the gene in the transformed cell;

(c) contacting the transformed cell with one or more compounds;

(d) measuring the detectable signal; and

(e) isolating or identifying an activator compound and/or an inhibitor compound by measuring the detectable signal.

2. An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of:

(a) a polynucleotide sequence represented by SEQ ID No;

161; and

(b) a polynucleotide sequence encoding a protein that activates NF-κ

B and that hybridizes under stringent conditions with a polynucleotide having a polynucleotide sequence complementary to the polynucleotide sequence of (a).

3. An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of:

(a) a nucleotide sequence represented by a coding region in SEQ ID NO;

161; and

(b) a nucleotide sequence encoding a protein that activates NF-κ

B and that hybridizes under stringent conditions with a polynucleotide having a polynucleotide sequence complementary to the polynucleotide sequence of (a).

4. An isolated polynucleotide comprising a nucleotide sequence which encodes a protein that activates NF-κ

B and has at least 95% identity to a polynucleotide having the sequence of the coding protein of SEQ ID NO;

161 over the entire length thereof.