METHODS AND COMPOSITIONS ASSOCIATED WITH ADMINISTRATION OF AN EXTRACT OF GANODERMA LUCIDUM

• US 20070105814A1
• Filed: 09/21/2006
• Published: 05/10/2007
• Est. Priority Date: 08/06/2001
• Status: Active Grant

• Current Assignee: Academia Sinica (Government of The Republic of China)
• Original Assignee: Academia Sinica (Government of The Republic of China)
• Inventors: Hsu, Hsien-Yeh, Wong, Chi-Huey, Hua, Kuo-Feng
• Granted Patent: US 7,687,064 B2
• Time in Patent Office: Days
• US Class Current: 514/54
• CPC Class Codes: A61K 36/074 Ganoderma

Abstract

The present disclosure provides methods for increasing the lipopolysaccharide-induced secretion of IL-1 by macrophages, for increasing serum levels of IL-1 in a mammal, for increasing the serum level of IL-1 receptor antagonist (IL-1Ra) in a mammal, for increasing the secretion of IL-1 by a monocyte, for increasing the secretion of IL-1Ra by a monocyte, for increasing the secretion of IL-1Ra by a macrophage, for increasing expression of TLR4 on the surface of a macrophage, for increasing expression of CD14 on the surface of macrophage, for increasing the uptake and clearance of lipopolysaccharide (LPS) by a LPS-stimulated macrophage, and for increasing lipopolysaccharide (LPS)-stimulated activation of at least one of ERK, JNK, and p38 in a macrophage. The methods of the disclosure involve the administration to mammals and immune cells of a fucose-containing glycoprotein fraction from Ganoderma lucidum.

Claims

1. A method for increasing the secretion of IL-1 by a lipopolysaccharide (LPS)-stimulated macrophage, the method comprising administering an effective amount of a fucose-containing glycoprotein fraction from Ganoderma lucidum to said macrophage prior to and/or during LPS stimulation.

2. A method for increasing the serum level of IL-1 that is produced in response to introduction of lipopolysaccharide (LPS) into a mammal, the method comprising administering an effective amount of a fucose-containing glycoprotein fraction from Ganoderma lucidum to said mammal prior to and/or during contact of said mammal with said LPS.

3. A method for increasing the serum level of IL-1 receptor antagonist (IL-1Ra) in a mammal, the method comprising administering an effective amount of a fucose-containing glycoprotein fraction from Ganoderma lucidum to said mammal.

4. A method for increasing the secretion of IL-1 by a monocyte, the method comprising administering an effective amount of a fucose-containing glycoprotein fraction from Ganoderma lucidum to said monocyte.

5. A method for increasing the secretion of IL-1Ra by a monocyte is disclosed, the method comprising administering an effective amount of a fucose-containing glycoprotein fraction from Ganoderma lucidum to said monocyte.

6. A method for increasing the secretion of IL-1Ra by a macrophage, the method comprising administering an effective amount of a fucose-containing glycoprotein fraction from Ganoderma lucidum to said macrophage.

7. A method for increasing expression of TLR4 on the surface of a macrophage, the method comprising administering an effective amount of a fucose-containing glycoprotein fraction from Ganoderma lucidum to said macrophage.

8. A method for increasing expression of CD14 on the surface of macrophage the method comprising administering an effective amount of a fucose-containing glycoprotein fraction from Ganoderma lucidum to said macrophage.

9. A method for increasing the uptake and clearance of lipopolysaccharide (LPS) by a LPS-stimulated macrophage, the method comprising administering an effective amount of a fucose-containing glycoprotein fraction from Ganoderma lucidum to said macrophage prior to and/or during LPS stimulation.

10. A method for increasing lipopolysaccharide (LPS)-stimulated activation of at least one of ERK, JNK, and p38 in a macrophage, the method comprising administering a fucose-containing glycoprotein fraction from Ganoderma lucidum to said macrophage prior to and/or during LPS stimulation.