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Small regulatory RNAs and methods of use

  • US 20070111227A1
  • Filed: 07/28/2006
  • Published: 05/17/2007
  • Est. Priority Date: 07/28/2005
  • Status: Abandoned Application
First Claim
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1. A method of identifying a full length small RNA from a signature sequence RNA molecule comprising:

  • a. providing a genomic DNA database;

    b. identifying said signature sequence of said small RNA molecule from said database using MPSS method, wherein said signature sequence comprises a portion of a full sequence of said small RNA molecule, wherein said small RNA molecule comprises about 15 to about 30 nucleotides;

    c. comparing said signature sequence to said genomic database;

    d. identifying one or more genomic regions that indicate identity with said signature sequence; and

    e. extending said signature sequence by a necessary number of nucleotides to obtain said full sequence of said small RNA molecule.

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