Device and method for multiple analyte detection

• US 20070111299A1
• Filed: 01/09/2007
• Published: 05/17/2007
• Est. Priority Date: 04/03/1996
• Status: Abandoned Application

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Applera Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Woudenberg, Timothy, Albin, Michael, Raysberg, Yefim, Ragusa, Robert, Kowallis, Reid, Winn-Deen, Emily
• Application Number:
  US11/651,318
• Publication Number:
  US 20070111299A1
• Time in Patent Office: Days
• US Class Current: 435/287.100
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00599 Solution-phase processes
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/0642 Filling fluids into wells by specific techniques
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 2400/049 vacuum
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/50851 specially adapted for heating or cooling samples laboratory heating apparatus B01L7/00; incubators C12M
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: C12Q 1/08 using multifield media
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 2565/631 being a biochannel or pore
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 50/08 Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: G01N 2035/00148 Test cards, e.g. Biomerieux or McDonnel multiwell test cards
• CPC Class Codes: G01N 21/6452 : Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: Y10S 436/805 : Optical property
• CPC Class Codes: Y10S 436/807 : Apparatus included in process claim, e.g. physical support structures
• CPC Class Codes: Y10S 436/808 : Automated or kit
• CPC Class Codes: Y10S 436/809 : Multifield plates or multicontainer arrays
• CPC Class Codes: Y10T 436/143333 : Saccharide [e.g., DNA, etc.]
• CPC Class Codes: Y10T 436/25 : including sample preparation

Abstract

The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device includes a substrate which defines a sample-distribution network having (i) a sample inlet, (ii) one or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of the chambers and the inlet. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.

Claims

1. A device comprising:

a substrate defining a sample-distribution network comprising

a sample inlet,

a plurality of detection chambers, and

a channel providing fluid communication between each of the plurality of detection chambers and the sample inlet; and

a detection reagent comprising enzyme substrate molecules designed to react with a selected analyte enzyme and pre-loaded in each of the plurality of detection chambers.

2. The device of claim 1, wherein the enzyme substrate molecules comprise a chromogenic substrate.

3. The device of claim 2, wherein the chromogenic substrate comprises a reducing dye.

4. The device of claim 2, wherein the chromogenic substrate is at least one of NAD/NADH, FAD/FADH, and nitrophenol.

5. The device of claim 1, wherein the detection reagent further comprises a carrier protein.

6. The device of claim 5, wherein the carrier protein comprises bovine serum albumin.

7. The device of claim 1, wherein the enzyme substrate molecules have been dried down.

8. The device of claim 1, wherein one or more detection chambers of the plurality of detection chambers comprise a control.

9. The device of claim 8, wherein the control comprises a selected reagent.

10. The device of claim 1, wherein the detection reagents in each chamber emit light at a first respective wavelength, each of the detection chambers of the plurality of detection chambers comprises a selected reagent therein, and the selected reagent emits light at a second wavelength that is distinctive from each first respective wavelength.

11. The device of claim 1, wherein the detection reagents in each chamber emit light at a first respective wavelength, each of the detection chambers of the plurality of detection chambers comprises a calibration standard therein, and the calibration standard emits light at a second wavelength that is distinctive from each first respective wavelength.

12. The device of claim 1, wherein each of the detection chambers of the plurality of detection chambers comprises a calibration standard therein.

13. A device comprising:

a substrate defining a sample-distribution network comprising

a sample inlet,

a plurality of detection chambers, and

a channel providing fluid communication between each of the plurality of detection chambers and the sample inlet; and

a detection reagent comprising drug target molecules pre-loaded in each of the plurality of detection chambers and designed to react with respective selected analyte drugs.

14. The device of claim 13, wherein the drug target molecules comprise a receptor protein.

15. The device of claim 13, wherein the drug target molecules pre-loaded in each of the plurality of detection chambers are designed to react with at least one of a receptor protein agonist and a receptor protein antagonist.

16. The device of claim 13, wherein the drug target molecules pre-loaded in each of the plurality of detection chambers are designed to react with at least one of a substrate, an activator, and an inhibitor of a drug target.

17. The device of claim 13, wherein the detection reagent further comprises a carrier protein.

18. The device of claim 17, wherein the carrier protein comprises bovine serum albumin.

19. The device of claim 13, wherein the drug target molecules have been dried down.

20. The device of claim 13, wherein one or more detection chambers of the plurality of detection chambers comprise a control.

21. The device of claim 20, wherein the control comprises a selected reagent.

22. The device of claim 13, wherein the detection reagents in each chamber emit light at a first respective wavelength, each of the detection chambers of the plurality of detection chambers comprises a selected reagent therein, and the selected reagent emits light at a second wavelength that is distinctive from each first respective wavelength.

23. The device of claim 13, wherein the detection reagents in each chamber emit light at a first respective wavelength, each of the detection chambers of the plurality of detection chambers comprises a calibration standard therein, and the calibration standard emits light at a second wavelength that is distinctive from each first respective wavelength.

24. The device of claim 13, wherein each of the detection chambers of the plurality of detection chambers comprises a calibration standard therein.

25. A device comprising:

a substrate defining a sample-distribution network comprising

a sample inlet,

a plurality of detection chambers, and

a channel providing fluid communication between each of the plurality of detection chambers and the sample inlet; and

thermally stable DNA polymerase pre-loaded in each of the plurality of detection chambers.

26. The device of claim 25, further comprising selected-sequence polynucleotides pre-loaded in each of the plurality of detection chambers.

27. The device of claim 25, wherein the thermally stable DNA polymerase has been dried down.

28. The device of claim 25, wherein one or more detection chambers of the plurality of detection chambers comprise a control.

29. The device of claim 28, wherein the control comprises a selected reagent.

30. The device of claim 25, wherein the detection reagents in each chamber emit light at a first respective wavelength, each of the detection chambers of the plurality of detection chambers comprises a selected reagent therein, and the selected reagent emits light at a second wavelength that is distinctive from each first respective wavelength.

31. The device of claim 25, wherein the detection reagents in each chamber emit light at a first respective wavelength, each of the detection chambers of the plurality of detection chambers comprises a calibration standard therein, and the calibration standard emits light at a second wavelength that is distinctive from each first respective wavelength.

32. The device of claim 25, wherein each of the detection chambers of the plurality of detection chambers comprises a calibration standard therein.

33. A device comprising:

a substrate defining a sample-distribution network comprising

a sample inlet,

a plurality of detection chambers, and

a channel providing fluid communication between each of the plurality of detection chambers and the sample inlet; and

a pathogen detection reagent pre-loaded in each of the plurality of detection chambers and designed to detect a pathogen.

34. The device of claim 33, wherein one or more detection chambers of the plurality of detection chambers comprise a control.

35. The device of claim 34, wherein the control comprises a selected reagent.

36. The device of claim 33, wherein the detection reagents in each chamber emit light at a first respective wavelength, each of the detection chambers of the plurality of detection chambers comprises a selected reagent therein, and the selected reagent emits light at a second wavelength that is distinctive from each first respective wavelength.

37. The device of claim 33, wherein the detection reagents in each chamber emit light at a first respective wavelength, each of the detection chambers of the plurality of detection chambers comprises a calibration standard therein, and the calibration standard emits light at a second wavelength that is distinctive from each first respective wavelength.

38. The device of claim 33, wherein one or more detection chambers of the plurality of detection chambers comprise a calibration standard.

39. A method comprising:

providing a device, the device comprising

a substrate defining a sample-distribution network comprising

a sample inlet,

a plurality of detection chambers, and

a channel providing fluid communication between each of the plurality of detection chambers and the sample inlet, and

a pathogen detection reagent pre-loaded in each of the plurality of detection chambers and designed to detect a pathogen;

loading a sample in the detection chambers; and

detecting a pathogen in the detection chambers.

40. The method of claim 39, wherein one or more detection chambers of the plurality of detection chambers comprise a control, and the method further comprises detecting a signal generated by the control.

41. The method of claim 40, wherein the control comprises a selected reagent.

42. The method of claim 39, wherein the detection reagents in each chamber emit light at a first respective wavelength, each of the detection chambers of the plurality of detection chambers comprises a selected reagent therein, the selected reagent emits light at a second wavelength that is distinctive from each first respective wavelength, and the method further comprises detecting the second wavelength and each first respective wavelength.

43. The method of claim 39, wherein the detection reagents in each chamber emit light at a first respective wavelength, each of the detection chambers of the plurality of detection chambers comprises a calibration standard therein, the calibration standard emits light at a second wavelength that is distinctive from each first respective wavelength, and the method further comprises detecting the second wavelength and each first respective wavelength.

44. The method of claim 39, wherein one or more detection chambers of the plurality of detection chambers comprise a calibration standard.