Detection of lysophosphatidylcholine for prognosis or diagnosis of a systemic inflammatory condition
First Claim
Patent Images
1. A method for the diagnosis or prognosis of a systemic inflammatory condition in a patient comprising the step of:
- measuring over time a plurality of amounts of total lysophosphatidylcholine in fluid or tissue of the patient to assess risk for the systemic inflammatory condition.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention provides methods and compositions useful for the diagnosis or prognosis of a systemic inflammatory condition such as sepsis.
-
Citations
71 Claims
-
1. A method for the diagnosis or prognosis of a systemic inflammatory condition in a patient comprising the step of:
measuring over time a plurality of amounts of total lysophosphatidylcholine in fluid or tissue of the patient to assess risk for the systemic inflammatory condition. - View Dependent Claims (2, 3, 4, 5, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
-
6. A method for the diagnosis or prognosis of a systemic inflammatory condition in a patient comprising the step of:
-
measuring over time a plurality of amounts of a compound according to formula (I) or a salt or solvate thereof, wherein R is C16-C18 acyl, in fluid or tissue of the patient to assess risk for the systemic inflammatory condition. - View Dependent Claims (7, 8, 9, 10, 11, 12)
-
- 29. A method for the diagnosis or prognosis of a systemic inflammatory condition in a patient comprising the step of comparing the amount of total lysophosphatidylcholine in fluid or tissue of the patient to a reference amount indicative of the amount of total lysophosphatidylcholine in fluid or tissue of an individual that has, or will have, the systemic inflammatory condition to assess risk for the systemic inflammatory condition.
-
30. A method for the diagnosis or prognosis of a systemic inflammatory condition in a patient comprising the step of:
-
comparing the amount of a compound according to formula (I) or a salt or solvate thereof, wherein R is C16-C18 acyl, in fluid or tissue of the patient to a reference amount indicative of the amount of the compound in fluid or tissue of an individual that has, or will have, the systemic inflammatory condition to assess risk for the systemic inflammatory condition.
-
-
41. A method for detecting lysophosphatidylcholine in a patient comprising the step of contacting a sample from fluid or tissue of the patient with:
-
an enzyme or reagent capable of reacting lysophosphatidylcholine to form glycerophosphatidylcholine;
an enzyme or reagent capable of reacting glycerophosphatidylcholine to form choline;
an enzyme or reagent capable of reacting choline, water and oxygen to form peroxide;
a peroxidase and a fluorogenic substrate of said peroxidase under conditions suitable for formation of a fluorescent product wherein the fluorescent product indicates lysophosphatidylcholine. - View Dependent Claims (42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54)
-
-
55. A method for the diagnosis or prognosis of a systemic inflammatory condition in a patient comprising the step of comparing the amount of total lysophosphatidylcholine in fluid or tissue of the patient to a reference amount indicative of the amounts of total lysophosphatidylcholine in fluids or tissues of a plurality of individuals that have, or will have, the systemic inflammatory condition to assess risk for the systemic inflammatory condition.
-
56. A method for the diagnosis or prognosis of a systemic inflammatory condition in a patient comprising the step of comparing the amount of total lysophosphatidylcholine in fluid or tissue of the patient to an internal standard in the fluid or the tissue to assess risk for the systemic inflammatory condition.
-
57. A method for the diagnosis or prognosis of a systemic inflammatory condition in a patient comprising the step of monitoring the amount of total lysophosphatidylcholine in fluid or tissue of the patient to assess risk for the systemic inflammatory condition.
-
58. A method for the diagnosis or prognosis of a systemic inflammatory condition in a patient comprising the step of evaluating the amount of total lysophosphatidylcholine in fluid or tissue of the patient to assess risk for the systemic inflammatory condition.
-
59. A method for monitoring treatment of a systemic inflammatory condition comprising the step of monitoring the amount of total lysophosphatidylcholine in fluid or tissue of a patient undergoing the treatment for the systemic inflammatory condition to assess the treatment.
-
60. A method for the diagnosis or prognosis of a systemic inflammatory condition in a patient comprising the step of
comparing the amount of a compound according to formula (I) or a salt or solvate thereof, wherein R is C16-C18 acyl, in fluid or tissue of the patient to a reference amount indicative of the amounts of the compound in fluids or tissues of a plurality of individuals that have, or will have, the systemic inflammatory condition to assess risk for the systemic inflammatory condition.
-
61. A method for the diagnosis or prognosis of a systemic inflammatory condition in a patient comprising the step of
monitoring the amount of a compound according to formula (I) or a salt or solvate thereof, wherein R is C16-C18 acyl, in fluid or tissue of the patient to assess risk for the systemic inflammatory condition.
-
62. A method for the diagnosis or prognosis of a systemic inflammatory condition in a patient comprising the step of
evaluating the amount of a compound according to formula (I) or a salt or solvate thereof, wherein R is C16-C18 acyl, in fluid or tissue of the patient to assess risk for the systemic inflammatory condition.
-
63. A method for monitoring treatment of a systemic inflammatory condition comprising the step of
monitoring the amount of a compound according to formula (I) or a salt or solvate thereof, wherein R is C16-C18 acyl, in fluid or tissue of a patient undergoing the treatment for the systemic inflammatory condition to assess the treatment.
- 64. A kit for diagnosis or prognosis, or monitoring, of a systemic inflammatory condition in a patient, said kit comprising a composition capable of use for measuring the amount of total lysophosphatidylcholine in fluid or tissue of the patient.
-
65. A kit diagnosis or prognosis, or monitoring, of a systemic inflammatory condition in a patient, said kit comprising
an enzyme or reagent capable of reacting lysophosphatidylcholine to form glycerophosphatidylcholine, an enzyme or reagent capable of reacting glycerophosphatidylcholine to form choline, an enzyme or reagent capable of reacting choline, water and oxygen to form peroxide, a peroxidase and a fluorogenic substrate of said peroxidase.
-
68. A kit for diagnosis or prognosis, or monitoring, of a systemic inflammatory condition in a patient, said kit comprising a composition capable of use for measuring the amount of a compound according to formula (I)
or a salt or solvate thereof, wherein R is C16-C18 acyl, in fluid or tissue of the patient.
-
70. A device for the diagnosis or prognosis of a systemic inflammatory condition wherein said device is capable of measuring the amount of a compound according to formula (I)
or a salt or solvate thereof, wherein R is C16-C18 acyl, in fluid or tissue of the patient and wherein said device is capable of comparing the amount to a reference amount indicative of the amount of the compound in fluid or tissue of an individual that has, or will have, the systemic inflammatory condition.
-
71. A system for the diagnosis or prognosis of a systemic inflammatory condition comprising a first device capable of measuring the amount of a compound according to formula (I)
or a salt or solvate thereof wherein R is C16-C18 acyl, in fluid or tissue of the patient and a second device capable of comparing the amount to a reference amount indicative of the amount of the compound in fluid or tissue of an individual that has, or will have, the systemic inflammatory condition to assess risk for the systemic inflammatory condition.
Specification