Microsystems that integrate three-dimensional microarray and multi-layer microfluidics for combinatorial detection of bioagent at single molecule level

• US 20070116607A1
• Filed: 11/23/2005
• Published: 05/24/2007
• Est. Priority Date: 11/23/2005
• Status: Abandoned Application

• Current Assignee: Pharmacom Microelectronics, Inc.
• Original Assignee: Pharmacom Microlelectronics Incorporated
• Inventors: Wang, William, Yi, Jun, Halmela, Maria, Ke, Sheng, Lahteenmaki, Pertti, Kihara, Kazuma
• Application Number:
  US11/287,049
• Publication Number:
  US 20070116607A1
• Time in Patent Office: Days
• US Class Current: 422/83
• CPC Class Codes: B01J 2219/00306 Reactor vessels in a multiple arrangement
• CPC Class Codes: B01J 2219/00322 the individual reactor vessels being arranged serially in stacks
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00578 electrophoric
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00668 Two-dimensional arrays within three-dimensional arrays
• CPC Class Codes: B01J 2219/00704 integrated with the reactor apparatus
• CPC Class Codes: B01J 2219/0072 Organic compounds
• CPC Class Codes: B01J 2219/0074 Biological products
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/028 Modular arrangements
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2300/0627 Sensor or part of a sensor is integrated
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: G01N 1/10 in the liquid or fluent state burettes, pipettes B01L3/02; sampling of ground water E02D1/06; metering by volume of fluids or fluent solid material G01F11/00, G01F13/00
• CPC Class Codes: G01N 2001/027 field kits / quick test kits
• CPC Class Codes: G01N 2001/028 : Sampling from a surface, swabbing, vaporising
• CPC Class Codes: G01N 2035/00158 : Elements containing microarrays, i.e. "biochip"

Abstract

Stand-alone microsystems adapted for performing combinatorial detection of bioagents at single molecule level wherein the microsystems are featured with three-dimensional microarray and multi-layer microfluidics to thereby provide high throughput screening and high content screening sufficient to allow for substantially real-time performance of the microsystem. Methods for detection of bioagents at a single molecule level or single organism level include providing a reconfigurable microsystem adapted for performing combinatorial detection of bioagents at a single molecule level and reconfiguring the reconfigurable microsystem for various environments.

Claims

1. A stand-alone microsystem adapted for performing combinatorial detection of bioagents at a single molecule level wherein the microsystem comprises a three-dimensional microarray component and a multi-layer microfluidics component to thereby provide high throughput screening and high content screening sufficient to allow for substantially real-time performance of the microsystem.

2. The microsystem of claim 1 wherein the microarray component and the microfluidics component are integrated using microsticks-in-column to thereby permit rapid circulation, completed reagent recycling and continuous functioning of the microsystem.

3. The microsystem of claim 1 wherein the microarray component and the microfluidics component are integrated using microspheres-in-column to thereby permit rapid circulation, completed reagent recycling and continuous functioning of the microsystem.

4. The microsystem of claim 1 wherein the microarray component and the microfluidics component are integrated using microspacers-in-column to thereby permit rapid circulation, completed reagent recycling and continuous functioning of the microsystem.

5. The microsystem of claim 1 wherein the bioagent is from the set consisting of DNA, RNA, a protein, a bacterium, and a virus.

6. The microsystem of claim 1 further comprising a sample collection interface operatively connected to the microfluidics component.

7. The microsystem of claim 6 wherein the sample collection interface is from a set consisting of a mini-syringe for scaled collection of liquid, a mini-pressure hose for volumetric breath of air, a mini-screw for penetration of solid matter, and a pin-tip for scratching of an object surface.

8. The microsystem of claim 6 further comprising a signal processing component having at least one mode of operation and operatively connected to the microarray component.

9. The microsystem of claim 8 further comprising a data reporting component operatively connected to the signal processing component.

10. The microsystem of claim 1 wherein the microsystem comprises a dual mode architecture adapted for simultaneously performing both genomic testing and proteomic testing to thereby reduce false negative and false positive results.

11. The microsystem of claim 1 wherein the bioagent is a cell and wherein the microsystem is adapted to provide for fractional separation and parallel sampling of content of the cell to thereby enable observation of functional related cellular entities and related molecules.

12. The microsystem of claim 1 wherein the bioagent is a plant cell and wherein the microsystem is adapted to measure transgenic materials of the plant cell.

13. A universal platform adapted for performing combinatorial detection of bioagents at a single molecule level wherein the system comprises:

a sample collection interface for collecting a sample;

a microfluidics component operatively connected to the sample collection interface;

a microarray component operatively connected to the microfluidics component;

a signal processing component having at least one mode operatively connected to the microarray component; and

a data reporting component operatively connected to the signal processing component.

14. The universal platform of claim 13 wherein the microfluidics component provides for molecule separation of the sample to a single cell or molecule.

15. The universal platform of claim 14 wherein the signal processing component provides for a cascaded process of signal amplification from weak-level molecule-molecule interaction to medium-level fluorescence generation to high-level optical/electronic conversion.

16. The universal platform of claim 14 wherein the data reporting component is adapted for digital reporting.

17. The universal platform of claim 13, wherein the microfluidics component, the microarray component, the signal processing component, and the data reporting component being implemented in a microsystem having a configuration from the set consisting of (a) a dual-mode genomic and proteomic testing configuration, (b) a clinic diagnostic configuration, (c) an E. coli detection configuration, (d) a virus identification configuration, (e) a food inspection configuration, (f) a pharmaceutical screening configuration, (g) an interior aerosol monitoring configuration, (h) an exterior aerosol monitoring configuration, (i) an odorant detection configuration, (j) a poison detection configuration, (k) a diet measurement configuration, (l) an explosives detection configuration, (m) a human smell detection configuration, (n) a forensic detection configuration, (O) a GMO detection configuration, (p) a warzone inspection configuration, (q) an underwater surveillance configuration, and (r) an open environment configuration.

18. A microsystem, comprising:

a sample selection and collection subsystem;

a sample separation and diffusion subsystem operatively connected to the sample selection and collection subsystem;

a detection and signaling subsystem operatively connected to the sample separation and diffusion subsystem; and

a data reporting subsystem operatively connected to the detection and signaling subsystem.

19. The microsystem of claim 18 wherein the sample selection and collection subsystem is adapted for raw plant sample collection and cellular extraction.

20. A method for detection of bioagents at a single molecule level or organism level, comprising:

providing a reconfigurable microsystem adapted for performing combinatorial detection of bioagents at a single molecule level;

reconfiguring the reconfigurable microsystem for an environment.

21. The method of claim 20 wherein the reconfigurable microsystem comprises a microarray component and a microfluidics component integrated with the microarray component.

22. The method of claim 20 wherein the microsystem further comprises a sample collection interface operative connected to the microfluidics component.

23. The method of claim 20 wherein the step of reconfiguring includes reconfiguring the sample collection interface for the environment.

24. A dual mode, genomic and proteomic, method for detection of transgenic material from within a plant, comprising:

collecting a sample of the plant using a sample collector;

retaining plant cells from the sample while removing portions of the sample which are not plant cells using microfluidics and at least one microarray; and

measuring transgenic materials in the plant cells.