Methods of isolation, expansion and differentiation of fetal stem cells from chorionic villus, amniotic fluid, and placenta and therapeutic uses thereof
First Claim
Patent Images
1. A method for producing a population of cells enriched for pluripotent fetal stem cells, comprising selecting c-kit positive cells from a chorionic villus, amniotic fluid, or placenta sample.
0 Assignments
0 Petitions
Accused Products
Abstract
The present invention is directed to pluripotent fetal stem cells derived from chorionic villus, amniotic fluid, and placenta and the methods for isolating, expanding and differentiating these cells, and their therapeutic uses such as manipulating the fetal stem cells by gene transfection and other means for therapeutic applications.
-
Citations
49 Claims
- 1. A method for producing a population of cells enriched for pluripotent fetal stem cells, comprising selecting c-kit positive cells from a chorionic villus, amniotic fluid, or placenta sample.
-
13. A method of proliferating a population of cells enriched for pluripotent fetal stem cells comprising:
-
(a) selecting at least one c-kit positive cell from a chorionic villus, amniotic fluid or placenta sample;
(b) introducing said at least one selected cell to a culture medium; and
(c) proliferating said at least one selected cell in the culture medium.
-
- 14. A method of differentiating c-kit positive pluripotent fetal stem cells comprising providing a chorionic villus, amniotic fluid or placenta sample and inducing differentiation of c-kit positive cells within said sample by exposing the sample to one or more differentiation-inducing agents.
-
16. A method of differentiating c-kit positive pluripotent fetal stem cells comprising:
-
(a) providing a chorionic villus, amniotic fluid or placenta sample;
(b) obtaining cells from said sample; and
(c) inducing differentiation of c-kit positive cells from step (b) within said sample by exposing said cells to one or more differentiation-inducing agents. - View Dependent Claims (17, 18)
-
-
19. A method for storing pluripotent fetal stem cells comprising the steps of:
-
(a) obtaining a chorionic villus, amniotic fluid or placenta sample from a human subject;
(b) isolating a substantially enriched population the c-kit positive pluripotent fetal stem cells from the sample; and
(c) cryopreserving the isolated substantially enriched population of c-kit positive pluripotent fetal stem cells.
-
-
21. An isolated pluripotent fetal stem cell which is c-kit positive.
- 22. A method of treating disease in a human comprising administering a substantially enriched population of pluripotent c-kit positive human fetal stem cells into an individual in need thereof.
-
23. A method of transplanting an enriched population of pluripotent c-kit positive human fetal stem cells into a human in need thereof.
-
24. A composition suitable for bonemarrow transplantation comprising an enriched population of pluripotent c-kit positive human fetal stem cells.
- 25. A method of treating disease in a human comprising administering to a human in need thereof a substantially enriched population of cells comprising pluripotent c-kit positive human fetal stem cells which have been differentiated to a lineage selected from osteogenic, hematopoietic, adipogenic, myogenic, hepatic, neurogenic and endothelial cell lineage.
-
28. A method of transplanting into a human in need thereof a substantially enriched population of cells comprising pluripotent c-kit positive human fetal stem cells which have been differentiated to a lineage selected from osteogenic, hematopoietic, adipogenic, myogenic, hepatic, neurogenic and endothelial phenotype.
-
29. A composition suitable for bonemarrow transplantation comprising a substantially enriched population of cells comprising pluripotent c-kit positive human fetal stem cells which have been differentiated to a lineage selected from osteogenic, hematopoietic, adipogenic, myogenic, hepatic, neurogenic and endothelial phenotype.
- 30. A method of obtaining a population of cells enriched for pluripotent fetal stem cells, comprising isolating a tissue specimen from the chorionic villus of a human placenta.
-
31. A method of obtaining a population of cells enriched for pluripotent fetal stem cells, comprising isolating a tissue specimen containing said cells from human placenta.
- 32. A method of obtaining a population of cells enriched for pluripotent fetal stem cells, comprising selecting c-kit positive cells from a chorionic villus.
- 33. A method of obtaining a population of cells enriched for pluripotent fetal stem cells, comprising selecting c-kit positive cells from amniotic fluid.
- 34. A method of obtaining a population of cells enriched for pluripotent fetal stem cells, comprising selecting c-kit positive cells from placenta.
-
35. A method of obtaining a population of cells enriched for pluripotent fetal stem cells, comprising the steps of:
-
(a) cryopreseverving a tissue specimen from the chorionic villus;
(b) thawing the cryopreserved specimen at a later date; and
(c) selecting c-kit positive cells.
-
-
36. A method of obtaining a population of cells enriched for pluripotent fetal stem cells, comprising the steps of:
-
(a) cryopreseverving a specimen of the amniotic fluid;
(b) thawing the cryopreserved specimen at a later date; and
(c) selecting c-kit positive cells.
-
-
37. A method of obtaining a population of cells enriched for pluripotent fetal stem cells, comprising the steps of:
-
(a) cryopreseverving a tissue specimen from a placenta;
(b) thawing the cryopreserved specimen at a later date; and
(c) selecting c-kit positive cells.
-
-
38. A method of producing a population of cells enriched for pluripotent fetal stem cells comprising,
(a) isolating c-kit positive cells from the chorionic villus; - and
(b) proliferating said cells in culture medium.
- and
-
39. A method of producing differentiated tissue comprising,
(a) providing a tissue specimen from chorionic villus; -
(b) culturing the tissue under conditions that cause c-kit positive cells to proliferate; and
(c) upon induction cause the c-kit positive cells to differentiate.
-
-
40. A method of producing differentiated tissue comprising,
(a) providing a tissue specimen from amniotic fluid; -
(b) culturing the tissue under conditions that cause c-kit positive cells to proliferate; and
(c) upon induction cause the c-kit positive cells to differentiate.
-
-
41. A method of producing differentiated tissue comprising,
(a) providing a tissue specimen from placenta; -
(b) culturing the tissue under conditions that cause c-kit positive cells to proliferate; and
(c) upon induction cause the c-kit positive cells to differentiate.
-
-
46. A tissue-engineered construct comprising a biocompatible polymer scaffold and isolated c-kit positive fetal stem cells.
-
47. A method of obtaining a population of cells enriched for pluripotent fetal stem cells, comprising isolating a tissue specimen from amniotic fluid.
-
48. A method of producing a population of cells enriched for pluripotent fetal stem cells comprising,
(a) isolating c-kit positive cells from the placenta; - and
(b) proliferating said cells in culture medium.
- and
-
49. A method of producing a population of cells enriched for pluripotent fetal stem cells comprising,
(a) isolating c-kit positive cells from the amniotic fluid; - and
(b) proliferating said cells in culture medium.
- and
Specification