Carriers having biological substance

• US 20070117119A1
• Filed: 09/01/2006
• Published: 05/24/2007
• Est. Priority Date: 03/05/1999
• Status: Active Grant

• Current Assignee: Mitsubishi Chemical Corporation (Mitsubishi Chemical Group Corp.)
• Original Assignee: Mitsubishi Rayon Company Limited (Mitsubishi Chemical Holdings Corporation)
• Inventors: Sumi, Toshinori, Yu, Fujio, Watanabe, Fumiaki, Takahashi, Atsushi, Fujii, Wataru, Akita, Takashi, Murase, Kei, Miyauchi, Haruko, Ishimaru, Teruta, Oogami, Nobuko, Morishita, Takeharu, Ito, Chiho, Maehara, Osamu, Makino, Takayuki, Uragaki, Toshitaka, Ikeda, Tadanobu
• Granted Patent: US 9,080,285 B2
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: D06M 15/13 Alginic acid or derivatives thereof
• CPC Class Codes: D06M 15/15 Proteins or derivatives thereof
• CPC Class Codes: D06M 15/263 of unsaturated carboxylic acids; Salts or esters thereof
• CPC Class Codes: D06M 15/285 of unsaturated carboxylic acid amides or imides
• CPC Class Codes: D06M 15/333 of vinyl acetate; Polyvinylalcohol
• CPC Class Codes: D06M 15/3562 containing nitrogen
• CPC Class Codes: D06M 15/53 Polyethers polyacetals D06M15/39
• CPC Class Codes: D06M 16/00 Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
• CPC Class Codes: D06M 23/00 Treatment of fibres, threads, yarns, fabrics or fibrous goods made from such materials, characterised by the process
• CPC Class Codes: D06M 23/02 Processes in which the treating agent is releasably affixed or incorporated into a dispensing means
• CPC Class Codes: Y10T 156/1052 with cutting, punching, tearing or severing
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

The present invention relates to biological substance-immobilized fibers wherein a biological substance is immobilized on a fiber, fibers retaining a biological substance-immobilized gel, and fiber alignments having bundles of the above-described fibers and slices of the same.

Claims

1-19. -19. (canceled)

20. A method for producing a fiber alignment slice having coordinates for each fiber unit thereof, comprising:

(a) cutting sequentially a fiber alignment to obtain a series of fiber alignment slices S(1), S(2), . . . S(h), . . . S(m);

(b) selecting any given slice S(h) from m number of slices and determining two-dimensional coordinates for each fiber unit contained in said slice S(h) based on the coordinate reference points in said slice S(h);

(c) determining the two-dimensional coordinates of each fiber unit contained in slice S(i) located close to said slice S(h) based on the coordinate data of slice S(h) obtained in step (b) and the coordinate reference points in said slice S(i); and

(d) repeating steps (b) and (c) to determine the two-dimensional coordinates of each fiber unit in said fiber alignment slice;

wherein said fiber alignment in (a) comprises hollow, porous, or porous hollow fibers each of which may incorporate one or more immobilized biological substance(s) and which fiber alignment is obtained by binding and immobilizing individual fibers, wherein the biological substance is immobilized directly on and/or in said fiber.

24. The method of claim 20, which comprises:

binding a plurality of hollow fibers to make an alignment;

introducing one or more biological substance(s) into the inner wall and/or hollow part(s) of each hollow fiber constituting said alignment and immobilizing the substance therein; and

slicing the said alignment in a direction intersecting with the fiber axis.

25. The method of claim 20, which comprises:

binding a plurality of porous hollow fibers to make an alignment;

introducing one or more biological substance(s) into the inner wall, hollow and/or porous part(s) of each porous hollow fiber constituting said alignment and immobilizing the substance therein; and

slicing the said alignment in a direction intersecting with the fiber axis.

26. The method according to claim 24, wherein the immobilization of a biological substance in the inner wall and/or hollow part(s) of each hollow fiber constituting an alignment is carried out by immersing the extended tip of each hollow fiber constituting said alignment into a solution containing a biological substance, and introducing said solution into the hollow part of each hollow fiber constituting said alignment.

27. The method according to claim 25, wherein the immobilization of a biological substance in the inner wall, hollow and/or porous part(s) of each porous hollow fiber constituting an alignment is carried out by immersing the extended tip of each porous hollow fiber constituting said alignment into a solution containing a biological substance, and introducing said solution into the hollow and/or porous part(s) of each porous hollow fiber constituting said alignment.

60. The method of claim 20, wherein the fibers are selected from the group consisting of hollow fibers incorporating an immobilized biological substance, porous fibers incorporating an immobilized biological substance, and porous hollow fibers incorporating an immobilized biological substance, wherein the biological substance is directly immobilized on the fiber, in the fiber, or both on and in the fiber.

61. The method of claim 20, wherein the fibers are fibers retaining a gel which incorporates one or more immobilized biological substance(s), whereby the biological substance is immobilized on the fiber, in the fiber, or both on and in the fiber.

62. The method of claim 20, wherein the fibers are selected from the group consisting of solid fibers, hollow fibers, porous fibers and hollow porous fibers.

63. The method of claim 20, wherein the fibers are solid fibers, and wherein the gel incorporating an immobilized biological substance is retained on a surface of the fibers.

64. The method of claim 20, wherein the fibers are hollow fibers, and wherein the gel incorporating an immobilized biological substance is retained in a hollow part of the fibers.

65. The method of claim 20, wherein the fibers are porous fibers, and wherein the gel incorporating an immobilized biological substance is retained in the pore(s) of the fibers.

66. The method of claim 20, wherein the fibers are porous hollow fibers, and wherein the gel incorporating an immobilized biological substance is retained in a hollow part and the pore(s) of the fibers.

67. The method of claim 20, wherein the one or more biological substance(s) is selected from a group consisting of:

(a) nucleic acid, amino acid, sugar or lipid;

(b) a polymer consisting of one or more kinds of ingredients from the substances stated in (a) above; and

(c) a substance interacting with substances stated in (a) or (b) above.

68. The method of claim 20, wherein the one or more biological substance(s) is nucleic acid.

69. The method of claim 20, wherein the one or more biological substance(s) is selected from a group consisting of:

(a) nucleic acid, amino acid, sugar or lipid;

(b) a polymer consisting of one or more kinds of ingredients from the substances stated in (a) above; and

(c) a substance interacting with substances stated in (a) or (b) above.

70. The method of claim 20, wherein the biological substance is nucleic acid.

71. The method of claim 20, wherein said fibers also have a pigment retained on the fiber, in the fiber or both on and in the fiber, by means of the gel.

72. The method of claim 20, wherein said fiber bundle contains 100 or more individual fibers.

73. The method of claim 20, wherein said fiber bundle contains 1000 to 10,000,000 individual fibers.

74. The method of claim 20, wherein said fiber bundle has a fiber density ranging from 100 to 1,000,000 fibers per cm².

75. The method of claim 20, wherein the thickness of the fibers is 1 mm or less.

21-23. -23. (canceled)

28. A method for producing a fiber alignment, which comprises:

applying tension to a fiber bundle arranged in accordance with a sequence pattern of interest, and

immobilizing said fiber bundle by filling resin among fibers of said fiber bundle to make a fiber alignment.

29. The method according to claim 28, wherein the sequence of a fiber bundle is formed by:

(a) passing fibers through a plurality of jigs having pores of the same pattern as a sequence pattern of interest; and

(b) widening the intervals between said jigs.

30. The method according to claim 29, wherein the jigs are support lines constituting networks obtained by longitudinal and transverse lines, or a perforated board.

31-59. -59. (canceled)