METHOD OF MANUFACTURING A DIAGNOSTIC TEST STRIP

• US 20070117171A1
• Filed: 09/11/2006
• Published: 05/24/2007
• Est. Priority Date: 09/13/2005
• Status: Abandoned Application

• Current Assignee: Home Diagnostics Incorporated
• Original Assignee: Home Diagnostics Incorporated
• Inventors: Popovich, Natasha, Wegner, Greta, Slomski, Dennis
• Application Number:
  US11/530,542
• Publication Number:
  US 20070117171A1
• Time in Patent Office: Days
• US Class Current: 435/14
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: G01N 33/521 Single-layer analytical elements
• CPC Class Codes: G01N 33/523 the element being adapted for a specific analyte
• CPC Class Codes: G01N 33/525 Multi-layer analytical elements
• CPC Class Codes: G01N 33/526 the element being adapted for a specific analyte

Abstract

A method for manufacturing a test strip is provided. The method comprises selecting a test strip substrate material and positioning the test strip substrate material a predetermined distance from a matrix material disposed on a second substrate, wherein at least one enzyme to be deposited on the test strip substrate and having glucose as an enzymatic substrate is held within the matrix material. A laser pulse is directed towards the matrix material to release at least a portion of the at least one enzyme having glucose as an enzymatic substrate from the matrix material and deposit the enzyme on the test strip substrate.

Claims

1. A method for manufacturing a test strip, comprising:

selecting a test strip substrate material;

positioning the test strip substrate material a predetermined distance from a matrix material disposed on a second substrate, wherein at least one enzyme to be deposited on the test strip substrate and having glucose as an enzymatic substrate is held within the matrix material; and

directing a laser pulse towards the matrix material to release at least a portion of the at least one enzyme having glucose as an enzymatic substrate from the matrix material and deposit the enzyme on the test strip substrate.

2. The method of claim 1, wherein the laser pulse passes through the second substrate before striking the matrix material.

3. The method of claim 2, wherein the second substrate includes at least one or fused quartz and fused silica.

4. The method of claim 1, wherein the enzyme includes glucose oxidase.

5. The method of claim 1, wherein the enzyme includes glucose dehydrogenase.

6. The method of claim 1, wherein the matrix further includes at least one of a buffer and a reaction mediator.

7. The method of claim 1, further including positioning a mask between the test strip substrate material and the second substrate material to define a region on the test strip substrate material where the enzyme having glucose as an enzymatic substrate is deposited.

8. The method of claim 1, wherein the matrix material includes a frozen material.

9. The method of claim 1, wherein the matrix material includes a polymer.

10. The method of claim 9 , wherein the polymer includes a polymer selected from the group including polybutyl methacrylate, polyvinylidene, and polylactic acid.

11. A method for manufacturing a test strip, comprising:

selecting a test strip substrate material;

positioning the test strip substrate material a predetermined distance from a matrix material disposed on a second substrate, wherein at least one electrode material to be deposited on the test strip substrate is held within the matrix material; and

directing a laser pulse towards the matrix material to release at least a portion of the at least one electrode material from the matrix material and deposit the electrode material on the test strip substrate.

12. The method of claim 11, wherein the laser pulse passes through the second substrate before striking the matrix material.

13. The method of claim 11, wherein the electrode material includes carbon.

14. The method of claim 11, wherein the electrode material includes at least one of gold and palladium.

15. The method of claim 11, further including positioning a mask between the test strip substrate material and the second substrate material to define a region on the test strip substrate material where the electrode material substrate is deposited.

16. A method for manufacturing a test strip, comprising:

selecting a test strip substrate material;

positioning the test strip substrate material a predetermined distance from a electrode matrix material disposed on a second substrate, wherein at least one electrode material to be deposited on the test strip substrate is held within the matrix material;

directing a laser pulse towards the electrode matrix material to release at least a portion of the at least one electrode material from the electrode matrix material and deposit the electrode material on the test strip substrate;

positioning the test strip substrate material a predetermined distance from an enzyme matrix material disposed on a third substrate, wherein at least one enzyme to be deposited on the test strip substrate and having glucose as an enzymatic substrate is held within the enzyme matrix material; and

directing a laser pulse towards the enzyme matrix material to release at least a portion of the is enzyme having glucose as an enzymatic substrate from the enzyme matrix material and deposit the enzyme on the test strip substrate.

17. The method of claim 16, wherein the enzyme includes glucose oxidase.

18. The method of claim 16, wherein the enzyme includes glucose dehydrogenase.

19. The method of claim 16, wherein the enzyme matrix material further includes at least one of a buffer and a reaction mediator.

20. The method of claim 16, wherein the electrode material includes carbon.

21. The method of claim 16, wherein the electrode material includes at least one of gold and palladium.

22. A method for manufacturing a test strip, comprising:

selecting a test strip substrate material; and

applying an aerosolized material including at least one enzyme having glucose as an enzymatic substrate to the test strip substrate.

23. The method of claim 22, wherein the enzyme includes glucose oxidase.

24. The method of claim 22, wherein the enzyme includes glucose dehydrogenase.

25. The method of claim 22, further including positioning a mask over the test strip substrate material to define a region of the test strip substrate on which the at least one enzyme having glucose as an enzymatic substrate will be applied.

26. A method for manufacturing a test strip, comprising:

selecting a test strip substrate material; and

applying an aerosolized material including at least one electrode material to the test strip substrate.

27. The method of claim 26, wherein the electrode material includes carbon.

28. The method of claim 26, wherein the electrode material includes at least one of gold and palladium.

29. The method of claim 26, further including positioning a mask over the test strip substrate material to define a region of the test strip substrate on which the enzyme will be applied.

30. A method for manufacturing a test strip, comprising:

selecting a test strip substrate material;

applying an aerosolized material including at least one electrode material to the test strip substrate; and

applying an aerosolized material including at least one enzyme having glucose as an enzymatic substrate to the test strip substrate.

31. The method of claim 30, wherein the enzyme includes glucose oxidase.

32. The method of claim 30, wherein the enzyme includes glucose dehydrogenase.

33. The method of claim 30, wherein the electrode material includes a carbon paste.

34. The method of claim 30, wherein the electrode material includes gold.