Large scale parallel immuno-based allergy test and device for evanescent field excitation of fluorescence
First Claim
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1. A device for detecting and characterizing an allergy, said device comprising:
- a sample chamber; and
an array of microcantilevers wherein microcantilevers comprising said array have affixed thereto antigen such that there is a different species of antigen for each allergy it is desired to detect, and different species of antigen are on different microcantilevers in said array, wherein the free ends of the microcantilevers project into the sample chamber.
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Abstract
This invention provides a device and methods for the rapid detection and/or diagnosis and/or characterization of one or more allergies (e.g., causes IgE mediated allergic reaction (immediate hypersensitvity) in a mammal (e.g., a human or a non-human mammal). In certain embodiments, the device comprises a microcantilever array where different cantilevers comprising the array bear different antigens. Binding of IgE to the antigen on a cantilever causes bending of the cantilever which can be readily detected.
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Citations
59 Claims
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1. A device for detecting and characterizing an allergy, said device comprising:
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a sample chamber; and
an array of microcantilevers wherein microcantilevers comprising said array have affixed thereto antigen such that there is a different species of antigen for each allergy it is desired to detect, and different species of antigen are on different microcantilevers in said array, wherein the free ends of the microcantilevers project into the sample chamber. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. A device for detecting the presence, absence, or quanity of a plurality of analytes, said device comprising:
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a sample chamber; and
an array of microcantilevers wherein micocantilevers comprising said array have affixed thereto binding moieties such that there is a different species of binding moiety that specifically or preferentially binds each species of analyte that is to be detected; and
different species of binding moiety are on different microcantilevers in said array, wherein the free ends of the microcantilevers project into the sample chamber. - View Dependent Claims (18, 19, 20, 21, 22, 23)
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24. A device for supporting a sample and for providing evanescent field excitation of fluorescence in total internal reflectance microscopy (TIRFM), said device comprising:
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a substantially planar optical waveguide comprising two substantially parallel surfaces; and
an active optical coupler affixed or juxtaposed to said waveguide such that light generated from said coupler enters said waveguide, where said active optical coupler is not a fluorophore. - View Dependent Claims (27, 28, 31, 32, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59)
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25. A device for supporting a sample and for providing evanescent field excitation of fluorescence in total internal reflectance microscopy (TIRFM), said device comprising:
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a substantially planar optical waveguide comprising two substantially parallel surfaces;
an active optical coupler affixed or juxtaposed to said waveguide such that light generated from said coupler enters said waveguide; and
an angle filter comprising a material whose refractive index is between that of the waveguide and air, where said angle filter is disposed on a surface of said waveguide to substantially reduce light propagating in the waveguide within a predetermined range of angles. - View Dependent Claims (29, 33)
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26. A device for supporting a sample and for providing evanescent field excitation of fluorescence in total internal reflectance microscopy (TIRFM), said device comprising:
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a substantially planar optical waveguide comprising two substantially parallel surfaces; and
a passive optical coupler affixed or juxtaposed to said waveguide such that light provided from said coupler enters said waveguide. - View Dependent Claims (30)
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Specification