Device and method for analyte sensing

• US 20070117223A1
• Filed: 01/22/2007
• Published: 05/24/2007
• Est. Priority Date: 01/07/2003
• Status: Active Grant

• Current Assignee: Ashok Gowda, Ralph Ballerstadt, Roger Mcnichols
• Original Assignee: Ashok Gowda, Ralph Ballerstadt, Roger Mcnichols
• Inventors: Gowda, Ashok, McNichols, Roger, Ballerstadt, Ralph
• Granted Patent: US 8,404,495 B2
• Time in Patent Office: Days
• US Class Current: 436/518
• CPC Class Codes: G01N 21/78 producing a change of colour
• CPC Class Codes: G01N 33/542 with steric inhibition or signal modification, e.g. fluorescent quenching
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing

Abstract

In one aspect, the present invention is a system, a device and a method for sensing the concentration of an analyte in a fluid or matrix. The analyte may be glucose or any other chemical of interest. The fluid or matrix may be, for example, the fluid in a bioreactor, a food or agricultural product, any fluid or matrix in the body of an animal, or any other fluid or matrix whose concentration of an analyte is under investigation. In one embodiment, the analyte sensing device includes a housing having an interior space. Contained within the housing and in the interior space is one or more analyte sensing component(s). The analyte sensing component, in one embodiment, includes one or more radiation converting element(s), for example, converting chromophores. The radiation converting element(s) are capable of converting or modifying radiation of one or more wavelengths into radiation of one or more different wavelengths. The conversion efficiency of the radiation converting element(s) is at least partially dependent on the local concentration of an analyte within the analyte sensing component.

Claims

1-80. -80. (canceled)

81. An analyte sensing device for sensing a concentration of analyte in a fluid, the analyte sensing device comprising:

a polymeric matrix; and

an analyte sensing component disposed within the matrix and including a first radiation converting component that is capable of converting radiation of a first wavelength to at least one different wavelength wherein the efficiency of conversion is dependent on the concentration of the analyte in the housing.

82. The analyte sensing device of claim 81 further including a housing that surrounds polymeric matrix.

83. The analyte sensing device of claim 82 wherein the housing includes a permeable or semi-permeable portion.

84. The analyte sensing device of claim 83 wherein the housing is comprised of PEG and/or derivatives thereof, calcium phosphate, polyurethane, alginate, regenerated cellulose acetate, chitosan, or combinations thereof.

85. The analyte sensing device of claim 82 further including covalent linkages connected between the analyte sensing component and the housing.

86. The analyte sensing device of claim 82 further including covalent linkages wherein the covalent linkages retain the analyte sensing component within the housing.

87. The analyte sensing device of claim 81 wherein the analyte sensing component is embedded throughout the polymeric matrix.

88. The analyte sensing device of claim 81 wherein the polymeric matrix surrounds the analyte sensing component.

89. The analyte sensing device of claim 81 further including a permeable or semi-permeable capsule, disposed in polymeric matrix, wherein the analyte sensing component is disposed in the capsule.

90. The analyte sensing device of claim 81 wherein analyte sensing component further includes an analyte-specific binding ligand.

91. The analyte sensing device of claim 90 wherein the analyte sensing component further includes a macroporous matrix layer wherein the analyte-specific binding ligand is bound to the surface of the macroporous matrix layer or embedded within the macroporous matrix layer.

92. The analyte sensing device of claim 90 wherein the analyte is glucose and wherein the first radiation converting chromophore includes agarose beads and the analyte-specific binding ligand includes a lectin.

93. The analyte sensing device of claim 90 wherein the lectin is Concanavalin A.

94. The analyte sensing device of claim 90 wherein the analyte-specific binding ligand is covalently labeled with or is in close proximity to the first radiation converting chromophore.

95. The analyte sensing device of claim 81 wherein the analyte sensing component further includes a second radiation converting chromophore that is capable of converting radiation of a second wavelength to at least one different wavelength wherein the efficiency of conversion is dependent on the concentration of the analyte in the housing.

96. The analyte sensing device of claim 81 wherein the analyte sensing component further includes a second radiation converting chromophore that is capable of converting radiation of a second wavelength to at least one different wavelength wherein the efficiency of conversion is independent or substantially independent of the concentration of the analyte in the housing.

97. The analyte sensing device of claim 81 wherein the device is capable of being implanted within subcutaneous tissue of an animal body.

98-101. -101. (canceled)