Immunophenotype and immunogenicity of human adipose derived cells
First Claim
1. An isolated adipose tissue-derived adult stromal (ADAS) cell exhibiting a non-immunogenic characteristic, wherein said cell has been passaged up to at least the second passage, further wherein said cell expresses a stem cell associated characteristic selected from the group consisting of human multidrug transporter (ABCG2) and aldehyde dehydrogenase (ALDH).
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Abstract
The present invention encompasses methods and compositions for generating an isolated adipose tissue-derived stromal cell exhibiting a low level of immunogenicity. The present invention encompasses methods and compositions for reducing an immune response associated with transplantation by administering the recipient with an amount of adipose tissue-derived stromal cells effective to reduce or inhibit host rejection and/or host versus graft disease.
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Citations
36 Claims
- 1. An isolated adipose tissue-derived adult stromal (ADAS) cell exhibiting a non-immunogenic characteristic, wherein said cell has been passaged up to at least the second passage, further wherein said cell expresses a stem cell associated characteristic selected from the group consisting of human multidrug transporter (ABCG2) and aldehyde dehydrogenase (ALDH).
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7. A method of treating a transplant recipient to reduce in said recipient an immune response of effector cells against an alloantigen, the method comprising:
- administering to a transplant recipient, an isolated adipose tissue-derived adult stromal (ADAS) cell exhibiting a non-immunogenic characteristic, wherein said ADAS cell has been passaged up to at least the second passage, further wherein said ADAS cell expresses a stem cell associated characteristic selected from the group consisting of human multidrug transporter (ABCG2) and aldehyde dehydrogenase (ALDH), in an amount effective to reduce an immune response of effector cells against an alloantigen, whereby in the transplant recipient said effector cells have a reduced immune response against said alloantigen.
- View Dependent Claims (8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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24. A method of reducing an immune response by an effector cell against an alloantigen, the method comprising:
- contacting an effector cell with an isolated adipose tissue-derived adult stromal (ADAS) cell exhibiting a non-immunogenic characteristic, wherein said ADAS cell has been passaged up to at least the second passage, further wherein said cell ADAS expresses a stem cell associated characteristic selected from the group consisting of human multidrug transporter (ABCG2) and aldehyde dehydrogenase (ALDH), in an amount effective to reduce an immune response by said effector cell against said alloantigen.
- View Dependent Claims (25)
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26. A method of isolating an adipose tissue-derived stromal (ADAS) cell from a population of cells derived from adipose tissue, the method comprising:
- providing an antibody specific for ABCG2;
contacting said population of adipose-derived cells with said antibody under conditions suitable for formation of an antibody-adipose tissue-derived stromal cell complex; and
substantially separating said antibody-adipose tissue-derived stromal cell complex from said population of adipose-derived cells;
thereby isolating said adipose tissue-derived stromal cell. - View Dependent Claims (27, 28, 29, 30)
- providing an antibody specific for ABCG2;
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31. A method of enriching adipose tissue-derived stromal cells from a population of adipose-derived cells, said method comprising:
- providing an antibody specific for ABCG2;
contacting said population of adipose-derived cells with said antibody under conditions suitable for formation of an antibody-adipose tissue-derived stromal cell complex; and
substantially separating said antibody-adipose tissue-derived stromal cell complex from said population of adipose-derived cells;
thereby isolating said adipose tissue-derived stromal cell. - View Dependent Claims (32, 33, 34, 35)
- providing an antibody specific for ABCG2;
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36. A method of identifying an adipose tissue-derived stromal (ADAS) cell positive for ALDH from a population of cells derived from adipose tissue, the method comprising:
- providing a cleavable substrate specific for ALDH to said population of cells, wherein said substrate when so present in an ALDH+cell is cleaved, further wherein said cleaved substrate emits a fluorescence thereby identifying an ALDH+ADAS cell.
Specification