Obtaining measurements of light transmitted through an assay test strip

• US 20070122914A1
• Filed: 11/30/2005
• Published: 05/31/2007
• Est. Priority Date: 11/30/2005
• Status: Abandoned Application

• Current Assignee: Alverix, Inc. (Becton, Dickinson & Co)
• Original Assignee: Alverix, Inc. (Becton, Dickinson & Co)
• Inventors: Curry, Bo
• Application Number:
  US11/290,056
• Publication Number:
  US 20070122914A1
• Time in Patent Office: Days
• US Class Current: 436/518
• CPC Class Codes: G01N 2035/00108 Test strips, e.g. paper
• CPC Class Codes: G01N 21/8483 Investigating reagent band test-element handling not specific to a test method G01N33/4875; analytical elements specific to chemical analysis of biological material G01N33/52; autometer with reagent band G01N35/04
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing

Abstract

Systems and methods of obtaining measurements of light transmitted through an assay test strip are described. In one aspect, a test strip is held. The top side of the test strip is illuminated with light. The illuminating light that is transmitted through the test strip and out from the bottom side of the test strip is detected. In another aspect, a diagnostic test system includes a detection system and a retainer. The detection system includes an optical detector that produces a measurement signal in response to light. The retainer holds a test strip so that the top side of the test strip is exposed for illumination and the bottom side of the test strip faces the optical detector.

Claims

1. (canceled)

2. A diagnostic test system, comprising:

a detection system comprising a first optical detector operable to produce a measurement signal in response to light;

a retainer configured to hold a test strip comprising a flow path for a fluid sample, a bottom side, and a top side opposite the bottom side and supporting a detection zone that has at least one measurement region coupled to the flow path, wherein the retainer is configured to hold the test strip so that the top side is exposed for illumination and the bottom side faces the first optical detector; and

a housing containing the detection system and the retainer and comprising a translucent window permitting light from outside the housing to illuminate the measurement region when the test strip is held by the retainer.

4. The system of claim 2, wherein the detection system comprises a second optical detector facing the bottom side of the test strip when the test strip is held by the retainer and operable to produce a measurement signal in response to light.

5. The system of claim 4, wherein the first and second optical detectors have respective fields of view containing different respective areas of the bottom side of the test strip when the test strip is held by the retainer.

7. The system of claim 5, wherein the fields of view of the first and second optical detectors correspond to respective areas of the bottom side of the test strip displaced from one another in a direction parallel to the flow path.

8. The system of claim 4, further comprising a data analyzer operable to quantify respective ones of the measurement signals produced by the first optical detector with respect to ones of the measurement signals produced by the second optical detector.

9. The system of claim 8, wherein the data analyzer is operable to derive a quantified value by evaluating a function that compares respective ones of the measurement signals produced by the first optical detector and ones of the measurement signals produced by the second optical detector.

10. The system of claim 2, wherein the retainer comprises a window that allows light that is transmitted through the test strip to reach the optical detector when the test strip is held by the retainer.

11. The system of claim 2, further comprising the test strip.

12. The system of claim 11, wherein the test strip is translucent with respect to light within a wavelength range detectable by the optical detector.

13. The system of claim 12, wherein the test strip comprises a labeling zone containing a labeling substance that binds a label to a target analyte, and at least one of the at least one measurement region contains an immobilized reagent that binds the target analyte, wherein the label absorbs or reflects light within the wavelength range.

14. The system of claim 2, further comprising a motor operable to move at least one of the detection system and the retainer relative to one another, a position encoder operable to produce a signal that tracks relative movement between the detection system and the retainer, and a data analyzer operable to correlate the measurement signal with at least one position along the test strip based on the signal produced by the position encoder.

3. (canceled)

6. A diagnostic test system, comprising:

a detection system comprising first and second optical detectors each operable to produce respective measurement signals in response to light;

a retainer configured to hold a test strip comprising a flow path for a fluid sample, a bottom side, and a top side opposite the bottom side and supporting a detection zone that has multiple measurement regions coupled to the flow path, wherein the retainer is configured to hold the test strip so that the top side is exposed for illumination and the bottom side faces the optical detector, wherein

the second optical detector faces the bottom side of the test strip when the test strip is held by the retainer,

the first and second optical detectors have respective fields of view containing different respective areas of the bottom side of the test strip when the test strip is held by the retainer, and

the retainer is configured to hold the test strip in multiple measurement positions relative to the detection system, wherein in each of the measurement positions the field of view of the first optical detector corresponds to an area of the bottom side of the test strip shadowed by a respective one of the measurement regions and the field of view of the second optical detector corresponds to an area of the bottom side of the test strip free of shadowing by any of the measurement regions; and

a data analyzer operable to quantify respective ones of the measurement signals produced by the first optical detector with respect to ones of the measurement signals produced by the second optical detector for each of the measurement positions.

22. The system of claim 6, further comprising an alignment system configured cooperatively with the detection system to guide movement of at least one of the retainer and the detection system into each of the measurement positions.

23. The system of claim 6, wherein the fields of view of the first and second optical detectors correspond to respective areas of the bottom side of the test strip displaced from one another in a direction parallel to the flow path.

24. The system of claim 6, further comprising a light source operable to illuminate the measurement region when the test strip is held by the retainer.

25. The system of claim 6, wherein the data analyzer is operable to derive a quantified value by evaluating a function that compares respective ones of the measurement signals produced by the first optical detector and ones of the measurement signals produced by the second optical detector.

26. The system of claim 6, wherein the retainer comprises a window that allows light that is transmitted through the test strip to reach the optical detector when the test strip is held by the retainer.

27. The system of claim 6, further comprising the test strip, wherein the test strip is translucent with respect to light within a wavelength range detectable by the optical detector, wherein the test strip comprises a labeling zone containing a labeling substance that binds a label to a target analyte, and at least one of the at least one measurement region contains an immobilized reagent that binds the target analyte, wherein the label absorbs or reflects light within the wavelength range.

29. The system of claim 6, further comprising a motor operable to move at least one of the detection system and the retainer relative to one another, a position encoder operable to produce a signal that tracks relative movement between the detection system and the retainer, and a data analyzer operable to correlate the measurement signal with at least one position along the test strip based on the signal produced by the position encoder.

15-21. -21. (canceled)