Engineered blood vessels
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Accused Products
Abstract
The present invention relates to engineered blood vessels and methods of making such vessels using matrices comprising endothelial and smooth muscle cells, or cells capable of differentiating into endothelial and smooth muscle cell lineages (e.g., stem cells, or the progenitors thereof).
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Citations
58 Claims
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1. (canceled)
- 2. A method of making an engineered blood vessel comprising an endothelial intimal layer surrounded by a smooth muscle medial layer, said method comprising contacting one or more mitogenic with one or more attractant factors or one or more mitoattractant factors or combinations thereof with a matrix, said matrix having incorporated therein at least ECs and SMCs, said matrix and cells being circumferentially positioned around a tubular support, said factors having been added to the inside of the tubular support, said support having allowed said factors to move from the inside of the tube to the ECs in the matrix, wherein said contacting results in the formation of said endothelial intimal layer surrounded by said smooth muscle medial layer
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7-12. -12. (canceled)
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15. (canceled)
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17-19. -19. (canceled)
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21-26. -26. (canceled)
- 28. A composition in vitro comprising a matrix containing incorporated ECs and SMCs, said matrix containing said incorporated cells being circumferentially positioned around a tubular support, said support allowing movement of mitogenic, attractant, and mitoattractant factors across the support to said ECs, said composition comprising one or more mitogenic with one or more attractant factors or one or more mitoattractant factors or combinations thereof within said support.
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33-38. -38. (canceled)
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41-45. -45. (canceled)
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47-52. -52. (canceled)
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53. An engineered blood vessel comprising an intimal layer of ECs incorporated in a matrix and a medial layer of SMCs incorporated in a matrix, said layers being circumferentially positioned around a tubular support.
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54. A pharmaceutical composition comprising an engineered blood vessel in a pharmaceutically acceptable carrier, said engineered vessel comprising an intimal layer of ECs incorporated in a matrix and a medial layer of SMCs incorporated in a matrix, said layers being circumferentially positioned around a tubular support.
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55. An in vitro composition comprising ECs and SMCs incorporated in a matrix circumferentially positioned around a tubular support, wherein one or more mitogenic with one or more attractant factors or one or more mitoattractant factors or combinations thereof capable of permeating the support are present within the support.
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56. (canceled)
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57. A method of culturing cells in a matrix comprising the steps of
a. combining ECs and SMCs in a matrix; -
b. growing ECs and SMCs in a matrix on the exterior surface of a tubular support, wherein the support allows movement of mitogenic, attractant, and mitoattractant factors from within the support to said ECs; and
c. allowing movement of one or more mitogenic with one or more attractant factors or one or more mitoattractant factors or combinations thereof present within the support to said ECs.
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58. (canceled)
Specification