Nucleotide analogs

• US 20070128614A1
• Filed: 12/06/2005
• Published: 06/07/2007
• Est. Priority Date: 12/06/2005
• Status: Active Grant

• Current Assignee: Fluidigm Corporation
• Original Assignee: Helicos Biosciences Corporation
• Inventors: Liu, David
• Granted Patent: US 7,476,734 B2
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C07H 19/04 Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
• CPC Class Codes: C07H 21/04 with deoxyribosyl as saccharide radical

Abstract

The invention provides nucleotide analogs for use in sequencing nucleic acid molecules.

Claims

1. A nucleotide analog having the structure:

2. The nucleotide analog of claim 1, wherein a label is attached to B by a linker.

3. The nucleotide analog of claim 2, wherein said label is an optically-detectable label.

4. The nucleotide analog of claim 3, wherein said optically-detectable label is a fluorescent label.

5. The nucleotide analog of claim 1, wherein B is selected from the group consisting of cytosine, uracil, thymine, adenine, guanine, and analogs thereof.

6. The nucleotide analog of claim 2, wherein said linker comprises a cleavable bond.

7. The nucleotide analog of claim 6, wherein said cleavable bond is a chemically cleavable bond.

8. The nucleotide analog of claim 6, wherein said cleavable bond is a photochemically cleavable bond.

9. The nucleotide analog of claim 8, wherein said photochemically cleavable bond is selected from the group consisting of o-nitrobenzyl and derivatives thereof.

10. The nucleotide analog of claim 6, wherein said cleavable bond is cleaved upon exposure to a pH from about 11.3 to about 12.3.

11. The nucleotide analog of claim 6, wherein said cleavable bond is cleaved upon exposure to a pH of about 11.8.

12. A nucleotide analog having the structure:

13. The nucleotide analog of claim 12, wherein said label is an optically-detectable label.

14. The nucleotide analog of claim 13, wherein said optically-detectable label is a fluorescent label.