Methods for determining nucleotide sequence information

• US 20070128615A1
• Filed: 04/28/2006
• Published: 06/07/2007
• Est. Priority Date: 12/29/2003
• Status: Abandoned Application

• Current Assignee: Intel Corporation
• Original Assignee: Intel Corporation
• Inventors: Su, Xing
• Application Number:
  US11/414,681
• Publication Number:
  US 20070128615A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12Q 1/6818 involving interaction of two or more labels, e.g. resonant energy transfer
• CPC Class Codes: C12Q 1/6874 involving nucleic acid arrays, e.g. sequencing by hybridisation
• CPC Class Codes: C12Q 2565/632 being a surface enhanced, e.g. resonance, Raman spectrometer

Abstract

Provided herein, is a nucleic acid sequencing method based on detection of Raman signatures of oligonucleotide probes. Raman signatures of individually captured nucleic acid probes, optionally labeled by a Raman label or a positively charged enhancer, are detected. The sequences of captured probes are used to identify the nucleotide sequences of captured probes and complementary target nucleic acids, which are then aligned and used to obtain nucleic acid sequence information. In another embodiment, a method is provided for determining a nucleotide occurrence at a target nucleotide position of a target nucleic acid, that utilizes binding of the target nucleic acid to a labeled oligonucleotide probe that binds to the target nucleic acid, wherein the labeled oligonucleotide probe includes a first label and a second label, the first label being capable of affecting an optical property of the second label.

Claims

1. A detection system comprising:

a) a Raman spectrometer comprising a light source;

b) a Raman active surface in optical communication with the light source; and

c) a population of Raman-active oligonucleotide probes comprising an undetectable oligonucleotide backbone associated with a positively charged enhancer, wherein the Raman-active oligonucleotide probes are deposited on the Raman active surface.

2. The method of claim 1, wherein the positively charged enhancer is an amine group enhancer.

3. The method of claim 1, wherein the Raman active surface is a biochip.