Method for preparing crosslinked polyelectrolyte multilayer films

• US 20070129792A1
• Filed: 11/29/2004
• Published: 06/07/2007
• Est. Priority Date: 11/28/2003
• Status: Abandoned Application

• Current Assignee: Institut National De La Sante Et De La Recherche Medicale (Inserm) (Allianz Global Investors GmbH), Centre National De La Recherche Scientifique, Université Louis Pasteur
• Original Assignee: Institut National De La Sante Et De La Recherche Medicale (Inserm) (Allianz Global Investors GmbH), Centre National De La Recherche Scientifique, Université Louis Pasteur
• Inventors: Schaaf, Pierre, Voegel, Jean-Claude, Picart, Catherine, Frisch, Benoit, Cuisinier, Frederic, Decher, Gero
• Application Number:
  US10/580,544
• Publication Number:
  US 20070129792A1
• Time in Patent Office: Days
• US Class Current: 623/1.460
• CPC Class Codes: C08J 2305/08 Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
• CPC Class Codes: C08J 2367/00 Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain of polyester-amides C08J2377/12; of polyester-imides C08J2379/08; Derivatives of such polymers
• CPC Class Codes: C08J 2389/00 Characterised by the use of proteins; Derivatives thereof
• CPC Class Codes: C08J 3/246 Intercrosslinking of at least two polymers
• CPC Class Codes: C08K 5/29 Compounds containing one or more carbon-to-nitrogen double bonds
• CPC Class Codes: Y10T 428/1334 Nonself-supporting tubular film or bag [e.g., pouch, envelope, packet, etc.]
• CPC Class Codes: Y10T 428/1393 Multilayer [continuous layer]
• CPC Class Codes: Y10T 428/31551 Of polyamidoester [polyurethane, polyisocyanate, polycarbamate, etc.]
• CPC Class Codes: Y10T 428/31725 Of polyamide

Abstract

The invention relates to methods for preparing crosslinked polyelectrolytes, in particular crosslinked polyelectrolytes multilayer films. The invention also relates to a method of coating a surface, and the obtained coated article.

Claims

1-36. -36. (canceled)

37. A method for preparing cross-linked polyelectrolyte multilayers films, wherein said method comprises the reaction of complementary functional groups carboxylic groups and amino groups, present in the polymers that constitute the multilayer film, in the presence of a coupling agent, as to form amide bonds.

38. The method according to claim 37, wherein the used polyelectrolyte multilayers are assembled via any complementary interaction, especially electrostatic attraction and hydrogen bridging.

39. The method according to claim 37, wherein the polyelectrolyte multilayers films are biocompatible.

40. The method according to claim 37, wherein said polyelectrolyte multilayers comprise at least one layer pair of cationic polyelectrolytes and anionic polyelectrolytes.

41. The method according to claim 37, wherein said polyelectrolyte multilayers comprise at least one layer pair of cationic polyelectrolytes and anionic polyelectrolytes and the number of said layer pairs is from 1 to 1000, preferably from 2 to 100, more preferably from 5 to 60.

42. The method according to claim 37, wherein said carboxylic groups and amino groups are attached by covalent bonds to polyelectrolytes.

43. The method according to claim 37, wherein the polymers that constitute the multilayer film comprise cationic polyelectrolytes which present free amino groups and anionic polyelectrolytes which present free carboxylic groups.

44. The method according to claim 37, wherein the polymers that constitute the multilayer film comprising anionic polyelectrolytes which present free carboxylic groups are selected in the group consisting of polyacrylic acid, polymethacrylic acid, acid, poly(D,L-glutamic) acid, polyuronic acid (alginic, galacturonic, glucuronic . . . ), glycosaminoglycans (hyaluronic acid, dermatan sulphate, chondroitin sulphate, heparin, heparan sulphate, and keratan sulphate), poly(D,L-aspartic acid), any combination, of the polyamino acids, and mixtures thereof.

45. The method according to claim 37, wherein the polymers that constitute the multilayer film comprising cationic polyelectrolytes which present free amino groups are selected in the group consisting of poly(D,L-lysine), poly(diallyldimethylammonium chloride), poly(allylamine), poly(ethylene)imine, chitosan, Poly(L-arginine), Poly(ornithine), Poly(D,L-hystidine), poly(mannoseamine, and other sugars) and more generally any combination of the polyamino acids and mixtures thereof.

46. The method according to claim 37, wherein the polyelectrolyte multilayers can further comprise polymers with different functional groups, including cationic (sulfonium, phosphonium, ammonium, hydroxylamine, hydrazide), anionic (including poly(styrene sulfonate), poly(phosphate), polynucleic acid . . . ) and neutral (including polyacrylamide, polyethylene oxyde, polyvinyl alcohol) polymers.

47. The method according to claim 37, wherein the polyelectrolyte multilayers comprise a variety of materials, preferably synthetic polyions (polymers presenting ions), biopolymers such as DNA, RNA, collagen, peptides (such as a RGD sequence, Melanoma stimulating Hormone, or buforin), proteins, and enzymes, cells, viruses, dendrimers, colloids, inorganic and organic particles, dyes, vesicles, nano(or micro)capsules, nano(or micro)particles, polyelectrolytes complexes, free or complexed drugs, cyclodextrins, and mixtures thereof.

48. The method according to claim 37, wherein the coupling agent is a carbodiimide compound.

49. The method according to claim 37, wherein the coupling agent is a compound of formula (I):

RN═

C═

NR′

wherein R and R′

, which are identical or different, represent an alkyl or aryl group, preferentially an C1-C8 alkyl group.

50. The method according to claim 49, wherein the coupling agent is 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC).

51. The method according to claim 37, wherein the coupling agent is a peptide-coupling agent.

52. The method according to claim 37, wherein the reaction of carboxylic groups and amino groups of the polyelectrolyte multilayers in the presence of a coupling agent is carried out also in the presence of N-hydroxysuccinimide compounds.

53. The method according to claim 37, wherein the reaction of carboxylic groups and amino groups of the polyelectrolyte multilayers in the presence of a coupling agent is carried out also in the presence of N-hydroxysulfo succinimide para-nitrophenol, or dimethylaminopyridine.

54. A method of coating a surface, comprising (1) sequentially depositing on a surface alternating layers of polyelectrolytes to provide a coated surface presenting complementary reactive groups:

amino and carboxylic groups, wherein a first (or conversely second) polymer is a cationic polyelectrolyte and a second (or conversely first) polymer is an anionic polyelectrolyte, and (2) reacting said complementary reactive groups of the coated surface in the presence of a coupling agent, as to form amide bonds between said complementary reactive groups.

55. The method according to claim 54, comprising (1) sequentially bringing a surface into contact with polyelectrolyte solutions thereby adsorbing alternated layers of polyelectrolytes to provide a coated surface presenting amino and carboxylic groups, wherein a first (or conversely second) polymer is a cationic polyelectrolyte and a second (or conversely first) polymer is an anionic polyelectrolyte, and (2) reacting amino and carboxylic groups of the coated obtained surface in the presence of a coupling agent, as to form amide bonds.

56. The method according to claim 54, wherein depositing on a surface alternating layers of polyelectrolytes includes dipping, dip-coating, rinsing, dip-rinsing, spraying, inkjet printing, stamping, printing and microcontact printing, wiping, doctor blading or spin coating.

57. The method according to claim 54, wherein the depositing process involves coating and rinsing steps.

58. The method according to claim 54, wherein the carboxylic groups and amino groups are attached by covalent bonds to polyelectrolytes.

59. The method according to claim 54, wherein anionic polyelectrolytes which present free carboxylic groups are selected in the group consisting of polyacrylic acid, polymethacrylic acid, acid, poly(D,L-glutamic) acid, polyuronic acid (alginic, galacturonic, glucuronic . . . ), glycosaminoglycans (hyaluronic acid dermatan sulphate, chondroitin sulphate, heparin, heparan sulphate, and keratan sulphate), poly(D,L-aspartic acid), any combination of the polyamino acids, and mixtures thereof.

60. The method according to claim 54, wherein cationic polyelectrolytes which present free amino groups are selected in the group consisting of poly(D,L-lysine), poly(diallyidimethylammonium chloride), poly(allylamine), poly(ethylene)imine, chitosan, Poly(L-arginine), Poly(ornithine), Poly(D,L-hystidine), poly(mannoseamine, and other sugars) and more generally any combination of the polyamino acids and mixtures thereof.

61. The method according to claim 54, wherein polyelectrolyte multilayers can further comprise polymers with different functional groups, including cationic (sulfonium, phosphonium, ammonium, hydroxylamine, hydrazide), anionic (including poly(styrene sulfonate), poly(phosphate), polynucleic acid . . . ) and neutral (including polyacrylamide, polyethylene oxyde, polyvinyl alcohol) polymers.

62. The method according to claim 54, wherein the coupling agent is a carbodiimide compound.

63. The method according to claim 62, wherein the coupling agent is a compound of formula (I):

RN═

C═

NR′

wherein R and R′

, which are identical or different, represent an alkyl or aryl group, preferentially an C1-C8 alkyl group.

64. The method according to claim 54, wherein the coupling agent is 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC).

65. The method according to claim 54, wherein the coupling agent is a peptide coupling agent.

66. The method according to claim 54, wherein step (2) is carried out also in the presence of N-hydroxysuccinimide compounds.

67. The method according to claim 54, wherein step (2) is carried out also in the presence of N-hydroxysulfo succinimide para-nitrophenol, or dimethylaminopyridine.

68. The method according to claim 54, wherein the coated surface of step (1) further comprises a variety of materials, including synthetic polyions (polymers presenting ions), biopolymers such as DNA, RNA, collagen, peptides (such as a RGD sequence, Melanoma stimulating Hormone, or buforin), proteins, and enzymes, cells, viruses, dendrimers, colloids, inorganic or organic particles, dyes, vesicles, nano(micro)capsules and nano(micro)particles, polyelectrolytes complexes, free or complexed drugs, cyclodextrins, and mixtures thereof.

69. A coated article obtained by a method according to claim 54.

70. A coated article obtained by a method according to claim 54, wherein said coated article is biocompatible.

71. A coated article obtained by a method according to claim 54, wherein said article is selected from the group consisting of blood vessel stents, angioplasty balloons, vascular graft tubing, prosthetic blood vessels, vascular shunts, heart valves, artificial heart components, pacemakers, pacemaker electrodes, pacemaker leads, ventricular assist devices, contact lenses, intraocular lenses, sponges for tissue engineering, foams for tissue engineering, matrices for tissue engineering, scaffolds for tissue engineering, biomedical membranes, dialysis membranes, cell-encapsulating membranes, drug delivery reservoirs, drug delivery matrices, drug delivery pumps, catheters, tubing, cosmetic surgery prostheses, orthopedic prostheses, dental prostheses, bone and dental implant, wound dressings, sutures, soft tissue repair meshes, percutaneous devices, diagnostic biosensors, cellular arrays, cellular networks, microfluidic devices, and protein arrays.

72. A coated article obtained by a method according to claim 54, wherein said coated article further comprises a variety of materials, including synthetic polyions, biopolymers such as DNA, RNA, collagen, peptides (such as a RGD sequence, Melanoma stimulating Hormone, or buforin), proteins, and enzymes, cells, viruses, dendrimers, colloids, inorganic and organic particles, vesicles, nano(micro)capsules and nano(micro)particles, dyes, vesicles, nano(micro)capsules and nano(micro)particles, polyelectrolytes complexes, free or complexed drugs, cyclodextrins, and mixtures thereof.